A galectin with quadruple-domain from bay scallop Argopecten irradians is involved in innate immune response

doi:10.1016/j.dci.2011.01.006

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	A galectin with quadruple-domain from bay scallop Argopecten irradians is involved in innate immune response
	Song, Xiaoyan 1,3; Zhang, Huan 1,2; Wang, Lingling 1; Zhao, Jianmin 1; Mu, Changkao 1,2; Song, Linsheng 1; Qiu, Limei 1; Liu, Xiaolin 3
	2011-05-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	35 期号:5 页码:592-602
文章类型	Article
摘要	Galectins are a family of p-galactoside-binding lectins that specifically bind to p-galactoside residues and play crucial roles in innate immune responses of invertebrates and vertebrates. The cDNA of bay scallop Argopecten irradians galectin (designated as AiGal2) was cloned by rapid amplification of cDNA ends (RACE) method based on the expressed sequence tag (EST). The full-length cDNA of AiGal2 was of 2137bp. The open reading frame encoded a polypeptide of 555 amino acids containing four carbohydrate-recognition domains. The deduced amino acid sequence and multi-domain organization of AiGal2 were highly similar to those of mollusk galectins. A typical galectin fold in beta-sandwich arrangement was identified in the potential tertiary structure of all the four CRDs in AiGal2. The mRNA transcripts of AiGal2 were found to be constitutively expressed in a wide range of tissues and mainly in hepatopancreas, adductor muscle and kidney. After scallops were challenged by Vibrio anguillarum or Micrococcus luteus, the mRNA expression level of AiGal2 was up-regulated significantly, while it did not changed remarkably after Pichia pastoris challenge. The recombined AiGal2 (rAiGal2) exhibited strong activity to agglutinate E coli, V. anguillarum, Vibrio fluvialis, Edwardsiella tarda and M. luteus, and the agglutinating activities could be inhibited by both D-galactose and lactose. The in vitro encapsulation assay revealed that rAiGal2 could bind to hemocytes and enhanced its encapsulation of agarose beads. These results collectively suggested that AiGal2 functioned as a pattern recognition receptor in immune defense and contributed to the non-self recognition and elimination in cellular immune response of bay scallop. (C) 2011 Elsevier Ltd. All rights reserved.; Galectins are a family of p-galactoside-binding lectins that specifically bind to p-galactoside residues and play crucial roles in innate immune responses of invertebrates and vertebrates. The cDNA of bay scallop Argopecten irradians galectin (designated as AiGal2) was cloned by rapid amplification of cDNA ends (RACE) method based on the expressed sequence tag (EST). The full-length cDNA of AiGal2 was of 2137bp. The open reading frame encoded a polypeptide of 555 amino acids containing four carbohydrate-recognition domains. The deduced amino acid sequence and multi-domain organization of AiGal2 were highly similar to those of mollusk galectins. A typical galectin fold in beta-sandwich arrangement was identified in the potential tertiary structure of all the four CRDs in AiGal2. The mRNA transcripts of AiGal2 were found to be constitutively expressed in a wide range of tissues and mainly in hepatopancreas, adductor muscle and kidney. After scallops were challenged by Vibrio anguillarum or Micrococcus luteus, the mRNA expression level of AiGal2 was up-regulated significantly, while it did not changed remarkably after Pichia pastoris challenge. The recombined AiGal2 (rAiGal2) exhibited strong activity to agglutinate E coli, V. anguillarum, Vibrio fluvialis, Edwardsiella tarda and M. luteus, and the agglutinating activities could be inhibited by both D-galactose and lactose. The in vitro encapsulation assay revealed that rAiGal2 could bind to hemocytes and enhanced its encapsulation of agarose beads. These results collectively suggested that AiGal2 functioned as a pattern recognition receptor in immune defense and contributed to the non-self recognition and elimination in cellular immune response of bay scallop. (C) 2011 Elsevier Ltd. All rights reserved.
关键词	Argopecten Irradians Galectin Innate Immunity Pattern Recognition Receptor Recombinant Protein Bacteria Agglutinating Activity Encapsulation
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2011.01.006
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000289333500010
引用统计	被引频次：71[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11745
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.NW A&F Univ, Yangling 712100, Shaanxi, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Song, Xiaoyan,Zhang, Huan,Wang, Lingling,et al. A galectin with quadruple-domain from bay scallop Argopecten irradians is involved in innate immune response[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(5):592-602.
APA	Song, Xiaoyan.,Zhang, Huan.,Wang, Lingling.,Zhao, Jianmin.,Mu, Changkao.,...&Liu, Xiaolin.(2011).A galectin with quadruple-domain from bay scallop Argopecten irradians is involved in innate immune response.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,35(5),592-602.
MLA	Song, Xiaoyan,et al."A galectin with quadruple-domain from bay scallop Argopecten irradians is involved in innate immune response".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 35.5(2011):592-602.

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