Comparative proteomic analysis of challenged Zhikong scallop (Chlamys farreri): A new insight into the anti-Vibrio immune response of marine bivalves

doi:10.1016/j.fsi.2011.10.014

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	Comparative proteomic analysis of challenged Zhikong scallop (Chlamys farreri): A new insight into the anti-Vibrio immune response of marine bivalves
	Huan, Pin; Wang, Hongxia; Liu, Baozhong
	2011-12-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	31 期号:6 页码:1186-1192
文章类型	Article
摘要	The current studies on molecular mechanism of bivalves interacting with bacteria are mainly on mRNA and recombinant protein levels. These works provide little information on natural proteins, which limit further understandings. In this study, we conducted a pioneer work to promote researches on the anti-Vibrio immune response of Zhikong Scallop Chlamys farreri through proteomic techniques. Firstly a reference map was constructed for the hepatopancreas of C. farreri. Totally 65 protein spots were included in the reference map, while 46 of them were identified. Gene ontology analysis revealed high activities of metabolism and immunity in hepatopancreas. Furthermore, hepatopancreas of C. Jarred injected with Vibrio harveyi at 24 h post-injection (hpi) were used for comparative proteomic analysis. Totally 27 differentially expressed proteins spots after challenge were screened: and 15 were successfully identified. These proteins include some immune-related proteins, metabolism enzymes, and new molecules which were not paid attentions in previous immunity studies in C. farreri. The results indicated that molecular chaperons and the antioxidant system are key elements in the anti-Vibrio immune response of hepatopancreas of C. farreri. The identification of new molecules provides indications for further studies. The results of this work provide a new insight into the anti-Vibrio immune response of marine bivalves. (C) 2011 Published by Elsevier Ltd.; The current studies on molecular mechanism of bivalves interacting with bacteria are mainly on mRNA and recombinant protein levels. These works provide little information on natural proteins, which limit further understandings. In this study, we conducted a pioneer work to promote researches on the anti-Vibrio immune response of Zhikong Scallop Chlamys farreri through proteomic techniques. Firstly a reference map was constructed for the hepatopancreas of C. farreri. Totally 65 protein spots were included in the reference map, while 46 of them were identified. Gene ontology analysis revealed high activities of metabolism and immunity in hepatopancreas. Furthermore, hepatopancreas of C. Jarred injected with Vibrio harveyi at 24 h post-injection (hpi) were used for comparative proteomic analysis. Totally 27 differentially expressed proteins spots after challenge were screened: and 15 were successfully identified. These proteins include some immune-related proteins, metabolism enzymes, and new molecules which were not paid attentions in previous immunity studies in C. farreri. The results indicated that molecular chaperons and the antioxidant system are key elements in the anti-Vibrio immune response of hepatopancreas of C. farreri. The identification of new molecules provides indications for further studies. The results of this work provide a new insight into the anti-Vibrio immune response of marine bivalves. (C) 2011 Published by Elsevier Ltd.
关键词	Chlamys Farreri Vibrio Hepatopancreas Proteome
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2011.10.014
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000298569700058
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11805
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Huan, Pin,Wang, Hongxia,Liu, Baozhong. Comparative proteomic analysis of challenged Zhikong scallop (Chlamys farreri): A new insight into the anti-Vibrio immune response of marine bivalves[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(6):1186-1192.
APA	Huan, Pin,Wang, Hongxia,&Liu, Baozhong.(2011).Comparative proteomic analysis of challenged Zhikong scallop (Chlamys farreri): A new insight into the anti-Vibrio immune response of marine bivalves.FISH & SHELLFISH IMMUNOLOGY,31(6),1186-1192.
MLA	Huan, Pin,et al."Comparative proteomic analysis of challenged Zhikong scallop (Chlamys farreri): A new insight into the anti-Vibrio immune response of marine bivalves".FISH & SHELLFISH IMMUNOLOGY 31.6(2011):1186-1192.

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