IOCAS-IR  > 海洋生物技术研发中心
Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas
Zhang LinLin; Li Li; Zhang GuoFan; Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
2012-09-01
发表期刊CHINESE SCIENCE BULLETIN
ISSN1001-6538
卷号57期号:25页码:3312-3319
文章类型Article
摘要Fibrinogen-related proteins (FREPs) are lectins with at least a fibrinogen-like domain. They are widespread in Mollusca and play an important role as immune pattern-recognition receptors. These diverse molecules provide interesting models for the study of the evolution of innate immunity. Although oysters are among the most studied mollusks, there have been few investigations of this gene family in oysters. This paper reports the cloning of the first oyster FREP, named CgFREP, from the Pacific oyster Crassostrea gigas. Five types of FREP (CgFREP-1 to CgFREP-5) were obtained based on a single pair of primers, confirming their high diversity. The proteins deduced from these FREPs contained 253-363 amino acids and were divided into three subfamilies based on sequence identities (cutoff point < 86%). The topological structure of the phylogenetic tree showed the five CgFREPs clustered together and were completely separated from the subgroup of FREPs obtained from Biomphalaria glabrata (BgFREPs). This implies that FREP diversity evolved before the BgFREPs branched off. An interesting aspect of the CgFREPs was their individual hypermutation. Ninety-three SNPs and five indels were characterized among CgFREPs. Some mutations (e.g. an insertion in CgFREP-5) could cause premature translation termination. Multiple alignments suggest recombination events might have occurred during exchanges between alleles, or by somatic diversification, or both. Individual sequence variability parallels the extraordinary complexity of the innate immune system, suggesting a potential capacity to recognize and eliminate many kinds of pathogens, even in a primitive system. The diversity of immune pattern-recognition receptors may offer an alternative strategy that functions in the absence of highly specific immune globulins, which only emerged in vertebrates.
关键词Crassostrea Gigas Freps Fibrinogen-related Proteins Recombination Oysters
学科领域Multidisciplinary Sciences
DOI10.1007/s11434-012-5155-6
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000307750500009
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/12245
专题海洋生物技术研发中心
通讯作者Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Zhang LinLin,Li Li,Zhang GuoFan,et al. Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas[J]. CHINESE SCIENCE BULLETIN,2012,57(25):3312-3319.
APA Zhang LinLin,Li Li,Zhang GuoFan,&Li, L .(2012).Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas.CHINESE SCIENCE BULLETIN,57(25),3312-3319.
MLA Zhang LinLin,et al."Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas".CHINESE SCIENCE BULLETIN 57.25(2012):3312-3319.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Sequence variability(1393KB) 限制开放使用许可浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang LinLin]的文章
[Li Li]的文章
[Zhang GuoFan]的文章
百度学术
百度学术中相似的文章
[Zhang LinLin]的文章
[Li Li]的文章
[Zhang GuoFan]的文章
必应学术
必应学术中相似的文章
[Zhang LinLin]的文章
[Li Li]的文章
[Zhang GuoFan]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。