| Three ferritin subunits involved in immune defense from bay scallop Argopecten irradians | |
| Li, Juan1,2; Li, Li1; Zhang, Shoudu1,2; Li, Jiale3,4; Zhang, Guofan1; Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. | |
| 2012-02-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| 卷号 | 32期号:2页码:368-372 |
| 文章类型 | Article |
| 摘要 | Ferritin is a ubiquitous protein that plays an important role in iron storage and iron-withholding strategy of innate immunity. In this study, three genes encoding different ferritin subunits were cloned from bay scallop Argopecten irradians (AiFer1, AiFer2 and AiFer3) by rapid amplification of cDNA ends (RACE) approaches based on the known ESTs. The open reading frames of the three ferritins are of 516 bp, 522 bp and 519 bp, encoding 171,173 and 172 amino acids, respectively. All the AiFers contain a putative Iron Regulatory Element (IRE) in their 5'-untranslated regions. The deduced amino acid sequences of AiFers possess both the ferroxidase center of mammalian H ferritin and the iron nucleation site of mammalian L ferritin. Gene structure study revealed two distinct structured genes encoding a ferritin subunit (AiFer3). Quantitative real-time PCR analysis indicated the significant up-regulation of AiFers in hemocytes after challenged with Listonella anguillarum, though the magnitudes of AiFer1 and AiFer2 were much higher than that of AiFer3. Taken together, these results suggest that AiFers are likely to play roles in both iron storage and innate immune defense against microbial infections. (C) 2011 Elsevier Ltd. All rights reserved. |
| 关键词 | Argopecten Irradians Ferritin Iron-withholding Strategy Gene Structure Immune Response |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2011.10.030 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300751800017 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12253 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China 4.Shanghai Univ, Inst E, Aquaculture Div, Shanghai 200041, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Juan,Li, Li,Zhang, Shoudu,et al. Three ferritin subunits involved in immune defense from bay scallop Argopecten irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(2):368-372. |
| APA | Li, Juan,Li, Li,Zhang, Shoudu,Li, Jiale,Zhang, Guofan,&Zhang, GF .(2012).Three ferritin subunits involved in immune defense from bay scallop Argopecten irradians.FISH & SHELLFISH IMMUNOLOGY,32(2),368-372. |
| MLA | Li, Juan,et al."Three ferritin subunits involved in immune defense from bay scallop Argopecten irradians".FISH & SHELLFISH IMMUNOLOGY 32.2(2012):368-372. |
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