A novel C1qDC protein acting as pattern recognition receptor in scallop Argopecten irradians

doi:10.1016/j.fsi.2012.05.032

IOCAS-IR > 实验海洋生物学重点实验室

	A novel C1qDC protein acting as pattern recognition receptor in scallop Argopecten irradians
	Wang, Leilei 1,2; Wang, Lingling 1; Kong, Pengfei 1,2; Yang, Jialong 1,2; Zhang, Huan 1; Wang, Mengqiang1 ; Zhou, Zhi 1; Qiu, Limei 1; Song, Linsheng 1; Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-08-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	33 期号:2 页码:427-435
文章类型	Article
摘要	The C1q domain containing (C1qDC) proteins refer to a family of proteins containing the versatile charge pattern recognition globular C1q domain in the C-terminus, which could bind various ligands including PAMPs and trigger a serial of immune response. In this study, a novel C1qDC protein was identified from Argopecten irradians (designated as AiC1qDC-2). Its full-length cDNA was of 1062 bp with an open reading frame of 720 bp encoding a polypeptide of 240 amino acids containing a typical gC1 q domain. This gC1q domain possessed the typical 10-stranded beta-sandwich fold with a jelly-roll topology common to all C1q family members, and shared high homology with most of the other identified gC1q domains. The mRNA transcripts of AiC1qDC-2 were mainly detected in hepatopancreas, and also marginally detectable in mantle, gonad, adductor, gill and hemocytes. Its relative expression level in hemocytes was significantly up-regulated after challenges of fungi Pichia pastoris GS115 (P < 0.05), Gram-positive bacteria Micrococcus luteus (P < 0.05) and Gram-negative bacteria Vibrio anguillarum (P < 0.05). The recombinant protein of AiC1qDC-2 (rAiC1qDC-2) could bind various PAMPs, including LPS, PGN, polyI:C, mannan, beta-1,3-glucan as well as Yeast-glucan, and displayed agglutinating activity to fungi P. pastoris GS115, Gram-positive bacteria Bacillus subtilis and Gram-negative bacteria Escherichia coli TOP10F as well as V. anguillarum. All these results indicated that AiC1qDC-2 could function as a pattern recognition receptor to recognize various PAMPs on different pathogens in the innate immune responses of scallop, and provided new clues to understand the role of invertebrate C1qDC proteins in the ancient complement system. (c) 2012 Elsevier Ltd. All rights reserved.
关键词	Argopecten Irradians C1qdc Protein Pattern Recognition Receptor Pamps Binding Microbial Agglutinating Activity
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2012.05.032
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000306632800031
引用统计	被引频次：50[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12262
专题	实验海洋生物学重点实验室
通讯作者	Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, Leilei,Wang, Lingling,Kong, Pengfei,et al. A novel C1qDC protein acting as pattern recognition receptor in scallop Argopecten irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(2):427-435.
APA	Wang, Leilei.,Wang, Lingling.,Kong, Pengfei.,Yang, Jialong.,Zhang, Huan.,...&Wang, LL .(2012).A novel C1qDC protein acting as pattern recognition receptor in scallop Argopecten irradians.FISH & SHELLFISH IMMUNOLOGY,33(2),427-435.
MLA	Wang, Leilei,et al."A novel C1qDC protein acting as pattern recognition receptor in scallop Argopecten irradians".FISH & SHELLFISH IMMUNOLOGY 33.2(2012):427-435.

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