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	A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection
	Sun, Y.1,2; Hu, Y-H.1; Liu, C-S.3; Sun, L.1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-12-01
发表期刊	LETTERS IN APPLIED MICROBIOLOGY
ISSN	0266-8254
卷号	55期号:6页码:420-426
文章类型	Article
摘要	Edwardsiella tarda and Streptococcus iniae are important fish pathogens. We have reported previously a live E. tarda vaccine based on the attenuated strain TX5RM and a S. iniae DNA vaccine based on the antigen Sia10. In this study, we examined the possibility of constructing a cross-genus vaccine by taking advantage of the residual infectivity of TX5RM and using it as a carrier host for the natural delivery of a S. iniae DNA vaccine. For this purpose, the recombinant TX5RM, TX5RMS10, was created, which harbours and retains stably the DNA vaccine plasmid pCS10 that expresses Sia10. When flounder were vaccinated with TX5RMS10 via oral and immersion routes, TX5RMS10 was detected in multiple tissues within 12-14 days postvaccination (p.v.). At 7 and 14 days p.v., expression of the DNA vaccine was detected in spleen, kidney and liver. Following E. tarda and S. iniae challenge at one and 2 months p.v., the vaccinated fish exhibited relative per cent survival rates of 69-83%. Immunological analysis indicated that TX5RMS10-vaccinated fish produced specific serum antibodies and exhibited enhanced expression of a wide range of immune genes.
关键词	Cross-protection Delivery Dna Vaccine Edwardsiella Tarda Streptococcus Iniae
学科领域	Biotechnology & Applied Microbiology ; Microbiology
DOI	10.1111/j.1472-765X.2012.03307.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000314225000004
引用统计	被引频次：5[WOS]   [WOS记录]     [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12271
专题	实验海洋生物学重点实验室
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Sun, Y.,Hu, Y-H.,Liu, C-S.,et al. A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection[J]. LETTERS IN APPLIED MICROBIOLOGY,2012,55(6):420-426.
APA	Sun, Y.,Hu, Y-H.,Liu, C-S.,Sun, L.,&Sun, L .(2012).A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection.LETTERS IN APPLIED MICROBIOLOGY,55(6),420-426.
MLA	Sun, Y.,et al."A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection".LETTERS IN APPLIED MICROBIOLOGY 55.6(2012):420-426.

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