IOCAS-IR  > 实验海洋生物学重点实验室
Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis
Guan XiangYu2; Zhang WeiJie3; Chi XiaoYuan3; Lin HanZhi3,4; Wang JinFeng3; Qin Song1; Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.
2012-09-01
发表期刊CHINESE SCIENCE BULLETIN
ISSN1001-6538
卷号57期号:25页码:3294-3299
文章类型Article
摘要To biosynthesize fluorescent Spirulina platensis (Sp) beta-phycocyanin (PC) in Escherichia coli, a BLASTP search for homologs of the cpeS gene, a chromophore lyase, was performed against the Synechocystis sp. PCC 6803 (S6) proteome. A highly homologous gene, slr2049, was obtained from the S6 genome. Sites 82 and 153 in beta-phycocyanin of Sp were modified by site-directed mutagenesis. Two recombinant expression vectors were constructed and transformed into E. coli BL21: (i) pCDF-cpcB (C153A)-slr2049-sll0583-ho1-pcyA; and (ii) pCDF-cpcB (C82I)-slr2049-sll0583-ho1-pcyA. Lyases encoded by the genes slr2049 and sll0583 catalyzed the linking of Sp 82 beta-PC to phycocyanobilin (PCB), and fluorescent CpcB (C153A)-PCB was generated. We present a strategy for the co-expression of multiple genes in a single expression vector to identify the function of an unknown gene. Recombinant phycobiliproteins produced on a large scale are promising fluorescent tags for diagnostics and pharmacology.
关键词Escherichia Coli Phycocyanin Fluorescence Recombinant
学科领域Multidisciplinary Sciences
DOI10.1007/s11434-012-5264-2
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000307750500007
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/12302
专题实验海洋生物学重点实验室
海洋生态与环境科学重点实验室
通讯作者Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.China Univ Geosci, Sch Ocean Sci, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Guan XiangYu,Zhang WeiJie,Chi XiaoYuan,et al. Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis[J]. CHINESE SCIENCE BULLETIN,2012,57(25):3294-3299.
APA Guan XiangYu.,Zhang WeiJie.,Chi XiaoYuan.,Lin HanZhi.,Wang JinFeng.,...&Qin, S .(2012).Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis.CHINESE SCIENCE BULLETIN,57(25),3294-3299.
MLA Guan XiangYu,et al."Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis".CHINESE SCIENCE BULLETIN 57.25(2012):3294-3299.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Combination of hydro(237KB) 限制开放使用许可浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Guan XiangYu]的文章
[Zhang WeiJie]的文章
[Chi XiaoYuan]的文章
百度学术
百度学术中相似的文章
[Guan XiangYu]的文章
[Zhang WeiJie]的文章
[Chi XiaoYuan]的文章
必应学术
必应学术中相似的文章
[Guan XiangYu]的文章
[Zhang WeiJie]的文章
[Chi XiaoYuan]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Combination of hydrodechlorination and biodegradation for the abatement of chlorophenols.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。