Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation | |
Cui Yulin1,2; Jiang Peng1; Wang Jinfeng1,2 ; Li Fuchao1; Chen Yingjie1,2; Zheng Guoting3; Qin Song1; Jiang, P (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
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| 2012-05-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 0254-4059 |
| 卷号 | 30期号:3页码:471-475 |
| 文章类型 | Article |
| 摘要 | Platymonas (Tetraselmis) subcordiformis is a unicellular marine green alga. It was found to be very sensitive to the herbicide Basta through a sensitivity test indicating it could be employed as a selective agent. The bar gene is a practicable and selectable marker gene. The vector containing the expression cassette of the bar gene was transferred to P. subcordiformis by both particle bombardment and glass-bead agitation and transformants were then selected using Basta. Finally, Southern blotting analysis indicated that the bar gene had been successfully integrated into the nuclear genome of P. subcordiformis using both of the transgenic techniques, with the transformation efficiency of the glass-bead method being slightly higher than that of particle bombardment. This is the first report on stable transformation of P. subcordiformis, and will improve fundamental research and enlarge application of this alga. |
| 关键词 | Bar Glass-bead Agitation Nuclear Transformation Particle Bombardment Platymonas Subcordiformis |
| 学科领域 | Limnology ; Oceanography |
| DOI | 10.1007/s00343-012-1093-z |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303680400014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12334 |
| 专题 | 实验海洋生物学重点实验室 海洋生态与环境科学重点实验室 |
| 通讯作者 | Jiang, P (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Cui Yulin,Jiang Peng,Wang Jinfeng,et al. Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(3):471-475. |
| APA | Cui Yulin.,Jiang Peng.,Wang Jinfeng.,Li Fuchao.,Chen Yingjie.,...&Jiang, P .(2012).Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(3),471-475. |
| MLA | Cui Yulin,et al."Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.3(2012):471-475. |
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