Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Sequencing and analysis of four BAC clones containing innate immune genes from the Zhikong scallop (Chlamys farreri) | |
Zhao, Cui1,2; Zhang, Tongwu3; Zhang, Xiaojun1; Hu, Songnian3; Xiang, Jianhai1 ; Zhang, XJ (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Shandong, Peoples R China.
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| 2012-07-01 | |
| 发表期刊 | GENE
 |
| ISSN | 0378-1119 |
| 卷号 | 502期号:1页码:9-15 |
| 文章类型 | Article |
| 摘要 | The sequencing of BAC clones (similar to 100 kb) can reveal some characteristics of a genome that are challenging to obtain based on short sequences. Additionally, although the immune genes of the Zhikong scallop (Chlamys farreri) have been studied widely, few analyses have been conducted at the DNA level. In this study, four C. farreri BAC clones containing innate immune genes, including hsp70, Igbp (lipopolysaccharide and beta-1,3-glucan binding protein), serine protease and a gene with an immunoglobulin-like domain, were sequenced and analyzed both to explore the genomic characteristics of C. farreri based on long DNA sequences and to promote the study of C. farreri immune genes at the DNA level. The total length of the four BACs was 389.98 kb. A total of 34 genes were predicted in these sequences, and several features of protein-coding regions in the C. farreri genome were inferred based on this information. Two LGBP genes Were located close together in a 22-kb region in one BAC clone, indicating the physical linkage of some immune genes in C. farreri. A cluster of membrane transport genes was also observed; these genes might play important roles in eliminating toxins in C. farreri, which lives as a filter feeder. Further analysis showed 15.43% of the BAC sequence was repetitive. Tandem repeats were the most abundant repeat type, followed by transposable elements. A total of 31 SSRs were predicted in the four BACs. An IS10 family transposon was identified, and a suspected regulatory non-coding RNA gene for this transposon (RNA-OUT) was observed to overlap with it complementarily. This work will promote future studies on the genomics, immune system and non-coding regions of C. farreri. (C) 2012 Elsevier B.V. All rights reserved. |
| 关键词 | Lgbp Repetitive Sequences Gene Cluster Membrane Transport Genes Transposon |
| 学科领域 | Genetics & Heredity |
| DOI | 10.1016/j.gene.2012.04.009 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000304898900002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12380 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, XJ (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Shandong, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Chinese Acad Sci, Beijing Inst Genom, Beijing, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, Cui,Zhang, Tongwu,Zhang, Xiaojun,et al. Sequencing and analysis of four BAC clones containing innate immune genes from the Zhikong scallop (Chlamys farreri)[J]. GENE,2012,502(1):9-15. |
| APA | Zhao, Cui,Zhang, Tongwu,Zhang, Xiaojun,Hu, Songnian,Xiang, Jianhai,&Zhang, XJ .(2012).Sequencing and analysis of four BAC clones containing innate immune genes from the Zhikong scallop (Chlamys farreri).GENE,502(1),9-15. |
| MLA | Zhao, Cui,et al."Sequencing and analysis of four BAC clones containing innate immune genes from the Zhikong scallop (Chlamys farreri)".GENE 502.1(2012):9-15. |
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