Two spliced variants of insulin-like androgenic gland hormone gene in the Chinese shrimp, Fenneropenaeus chinensis

doi:10.1016/j.ygcen.2012.04.010

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	Two spliced variants of insulin-like androgenic gland hormone gene in the Chinese shrimp, Fenneropenaeus chinensis
	Li, Shihao 1,2; Li, Fuhua 1; Sun, Zheng 3; Xiang, Jianhai1 ; Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-06-01
发表期刊	GENERAL AND COMPARATIVE ENDOCRINOLOGY
ISSN	0016-6480
卷号	177 期号:2 页码:246-255
文章类型	Article
摘要	More and more evidence indicates that the insulin-like androgenic gland hormone (JAG) plays an important role in male sexual differentiation in crustaceans. In the present study, two JAG isoforms (Fc-IAG1 and Fc-IAG2) were identified from the penaeid shrimp Fenneropenaeus chinensis. Sequence analysis of JAG gene (Fc-IAG) showed that Fc-IAG1 and Fc-IAG2 were generated by alternative splicing of Fc-IAG pre-mRNA, and they shared almost the same deduced amino acid sequence. Both of them were composed of signal peptide, B chain, C peptide and A chain. They both contained the six conserved cysteine residues and a putative N-linked glycosylated site like IAGs reported in other crustacean species. Tissue distribution and in situ hybridization analysis revealed that they had the highest expression level in the androgenic gland. The transcripts of Fc-IAG1 and Fc-IAG2 could also be detected in hepatopancreas and nerve cord of both sexes at a low expression level. Analysis on their temporal expression profiles showed that they expressed at all embryonic and post-larvae stages. The expression of Fc-IAG1 at different developmental stages displayed a low and stable manner, while the expression of Fc-IAG2 began to increase from post-larvae stages, which suggested that Fc-IAG2 might be involved in male sexual differentiation. In the 5' flanking sequence of Fc-IAG, putative binding sites for transcription factors regulating transcription of hormone genes and genes related to sexual development were predicted, which provided us a primary understanding on the regulation mechanism of Fc-IAG gene. This is the first time to report the gene structure of IAG gene and distinct variants of IAG transcripts in crustaceans. (c) 2012 Elsevier Inc. All rights reserved.
关键词	Androgenic Gland Hormone Gene Structure Gene Expression Alternative Splicing
学科领域	Endocrinology & Metabolism
DOI	10.1016/j.ygcen.2012.04.010
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000305305000005
引用统计	被引频次：70[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12404
专题	实验海洋生物学重点实验室
通讯作者	Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Li, Shihao,Li, Fuhua,Sun, Zheng,et al. Two spliced variants of insulin-like androgenic gland hormone gene in the Chinese shrimp, Fenneropenaeus chinensis[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2012,177(2):246-255.
APA	Li, Shihao,Li, Fuhua,Sun, Zheng,Xiang, Jianhai,&Li, FH .(2012).Two spliced variants of insulin-like androgenic gland hormone gene in the Chinese shrimp, Fenneropenaeus chinensis.GENERAL AND COMPARATIVE ENDOCRINOLOGY,177(2),246-255.
MLA	Li, Shihao,et al."Two spliced variants of insulin-like androgenic gland hormone gene in the Chinese shrimp, Fenneropenaeus chinensis".GENERAL AND COMPARATIVE ENDOCRINOLOGY 177.2(2012):246-255.

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