Variation in Rubisco and other photosynthetic parameters in the life cycle of Haematococcus pluvialis

doi:10.1007/s00343-012-1060-8

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	Variation in Rubisco and other photosynthetic parameters in the life cycle of Haematococcus pluvialis
	Chen Zhangfan 1,2; Wang Guangce 1; Niu Jianfeng 1; Wang, GC (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China.
	2012
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	30 期号:1 页码:136-145
文章类型	Article
摘要	Cells of Haematococcus pluvialis Flot. et Will were collected in four different growth phases. We quantified the initial and total enzyme activity of ribulose-1,5-bisphosphate carboxylase (Rubisco) in crude extracts, and the relative expression of large-subunit ribulose-1,5-bisphosphate caboxylase / oxygenase (rbcL) mRNA. We measured the ratio of photosynthetic rate to respiration rate (P/R), maximal effective quantum yield of photosystem II (F(v)/F(m)), electron transport rate (ETR), actual photochemical efficiency of PSII in the light (Phi PSII), and non-photochemical quenching (NPQ). Green vegetative cells were found to be in the most active state, with a relatively higher P/R ratio. These cells also displayed the lowest NPQ and the highest F(v)/F(m), ETR, and Phi PSII, indicating the most effective PSII. However, both Rubisco activity and rbcL mRNA expression were the lowest measured. In orange resting cysts with relatively lower P/R and NPQ, Rubisco activity and rbcL expression reached a peak, while F(v)/F(m), ETR, and Phi PSII were the lowest measured. Taking into account the methods of astaxanthin induction used in industry, we suggest that Rubisco may participate in astaxanthin accumulation in H. pluvialis. A continuous and sufficient supply of a carbon source such as CO(2) may therefore aid the large scale production of astaxanthin.
关键词	Haematococcus Pluvialis Rubisco Astaxanthin Life Cycle Growth Phase
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-012-1060-8
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000299040100013
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12405
专题	实验海洋生物学重点实验室
通讯作者	Wang, GC (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Chen Zhangfan,Wang Guangce,Niu Jianfeng,et al. Variation in Rubisco and other photosynthetic parameters in the life cycle of Haematococcus pluvialis[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(1):136-145.
APA	Chen Zhangfan,Wang Guangce,Niu Jianfeng,&Wang, GC .(2012).Variation in Rubisco and other photosynthetic parameters in the life cycle of Haematococcus pluvialis.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(1),136-145.
MLA	Chen Zhangfan,et al."Variation in Rubisco and other photosynthetic parameters in the life cycle of Haematococcus pluvialis".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.1(2012):136-145.

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