Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The modulation of extracellular superoxide dismutase in the specifically enhanced cellular immune response against secondary challenge of Vibrio splendidus in Pacific oyster (Crassostrea gigas) | |
Liu, Conghui1,3 ; Zhang, Tao1,3; Wang, Lingling1; Wang, Mengqiang1; Wang, Weilin1,3; Jia, Zhihao1,3; Jiang, Shuai1; Song, Linsheng2
| |
| 2016-10-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 卷号 | 63页码:163-170 |
| 文章类型 | Article |
| 摘要 | Extracellular superoxide dismutase (EcSOD) is a copper-containing glycoprotein playing an important role in antioxidant defense of living cells exposed to oxidative stress, and also participating in microorganism internalization and cell adhesion in invertebrates. EcSOD from oyster (designated CgEcSOD) had been previously reported to bind lipopolysaccharides (LPS) and act as a bridge molecule in Vibrio splendidus internalization. Its mRNA expression pattern, PAMP binding spectrum and microorganism binding capability were examined in the present study. The mRNA expression of CgEcSOD in hemocytes was significantly up-regulated at the initial phase and decreased sharply at 48 h post V. splendidus stimulation. The recombinant CgEcSOD protein (rCgEcSOD) could bind LPS, PGN and poly (I:C), as well as various microorganisms including Micrococcus luteus, Staphylococcus aureus, Escherichia coli, Vibrio anguillarum, V splendidus, Pastoris pastoris and Yarrowia lipolytica at the presence of divalent metal ions Cu2+. After the secondary V. splendidus stimulation, the mRNA and protein of CgEcSOD were both down regulated significantly. The results collectively indicated that CgEcSOD could not only function in the immune recognition, but also might contribute to the immune priming of oyster by inhibiting the foreign microbe invasion through a specific down-regulation. (C) 2016 Elsevier Ltd. All rights reserved. |
| 关键词 | Extracellular Superoxide Dismutase Rgd Motif Crassostrea Gigas Inhibiting Factor Immune Recognition Immune Priming |
| DOI | 10.1016/j.dci.2016.06.002 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000380623300018 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/130891 |
| 专题 | 实验海洋生物学重点实验室 海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, Conghui,Zhang, Tao,Wang, Lingling,et al. The modulation of extracellular superoxide dismutase in the specifically enhanced cellular immune response against secondary challenge of Vibrio splendidus in Pacific oyster (Crassostrea gigas)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,63:163-170. |
| APA | Liu, Conghui.,Zhang, Tao.,Wang, Lingling.,Wang, Mengqiang.,Wang, Weilin.,...&Song, Linsheng.(2016).The modulation of extracellular superoxide dismutase in the specifically enhanced cellular immune response against secondary challenge of Vibrio splendidus in Pacific oyster (Crassostrea gigas).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,63,163-170. |
| MLA | Liu, Conghui,et al."The modulation of extracellular superoxide dismutase in the specifically enhanced cellular immune response against secondary challenge of Vibrio splendidus in Pacific oyster (Crassostrea gigas)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 63(2016):163-170. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| The modulation of ex（1182KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论