Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus

doi:10.1007/s00343-016-5091-4

IOCAS-IR > 实验海洋生物学重点实验室

	Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus
	Peng Limin1,2,3 ; Zheng Yuan 1,2; You Feng 1,2; Wu Zhihao 1,2; Zou Yuxia 1,2; Zhang Peijun 1,2
	2016-09-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号	34 期号:5 页码:1054-1063
文章类型	Article
摘要	The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25 degrees C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical "S" shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.
关键词	Olive Flounder Paralichthys Olivaceus Testis Sertoli Cell Chromosome Fasl Transfection
DOI	10.1007/s00343-016-5091-4
收录类别	SCI
语种	英语
WOS记录号	WOS:000379737000017
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/130938
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Peng Limin,Zheng Yuan,You Feng,et al. Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(5):1054-1063.
APA	Peng Limin,Zheng Yuan,You Feng,Wu Zhihao,Zou Yuxia,&Zhang Peijun.(2016).Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(5),1054-1063.
MLA	Peng Limin,et al."Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.5(2016):1054-1063.

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