Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Crystal structures of the isochorismatase domains from Vibrio anguillarum | |
Du, Jiansen1,2; Deng, Tian1,2; Ma, Qingjun1,2 | |
2017-08-26 | |
发表期刊 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS |
卷号 | 490期号:3页码:827-833 |
文章类型 | Article |
摘要 | Antibiotic resistance is becoming a global threat and overuse of antibiotics in aquaculture disease control worsens the situation. To reduce the risk of drug resistance developed in aquaculture, safer biocontrol programs are needed. Antivirulence therapy, with less chance for developing drug resistance, is a promising approach. To facilitate antivirulence inhibitor design against Vibrio anguillarum, a serious aquaculture pathogen, we present crystal structures for isochorismatase domains of AngB and VabB, which are required to synthesize siderophore, a critical virulence factor. Both structures are highly similar to known isochorismatases in fold and active site, therefore we conclude inhibitors for isochorismatases can be developed in a common framework. The structural information will improve design of virulence inhibitors against Vibrio anguillarum. We also firstly report that isochorismatase family could bind endogenous metabolite during the hetero-expression process, which is likely nicotinic acid, nicotinamide or pyrazinic acid, based on structural analysis and affinity prediction. Taken together, our results provide precise structural information of isochorismatase domains for antivirulence inhibitor design against Vibrio anguillarum. (C) 2017 Elsevier Inc. All rights reserved. |
关键词 | Vibrio Isochorismatase Unknown Electron Density Antivirulence Inhibitor Aquaculture Disease |
DOI | 10.1016/j.bbrc.2017.06.125 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000406820600037 |
引用统计 | |
文献类型 | 期刊论文 |
版本 | 出版稿 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/143088 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Du, Jiansen,Deng, Tian,Ma, Qingjun. Crystal structures of the isochorismatase domains from Vibrio anguillarum[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2017,490(3):827-833. |
APA | Du, Jiansen,Deng, Tian,&Ma, Qingjun.(2017).Crystal structures of the isochorismatase domains from Vibrio anguillarum.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,490(3),827-833. |
MLA | Du, Jiansen,et al."Crystal structures of the isochorismatase domains from Vibrio anguillarum".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 490.3(2017):827-833. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Crystal structures o(2427KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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