Characterization and expression of StAR2a and StAR2b in the olive flounder Paralichthys olivaceus

doi:10.1016/j.gene.2017.05.008

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization and expression of StAR2a and StAR2b in the olive flounder Paralichthys olivaceus
	Liang, Dongdong1,2,3 ; Fan, Zhaofei1,2,3 ; Weng, Shenda 1,2; Jiao, Shuang 1,2; Wu, Zhihao 1,2; Zou, Yuxia 1,2; Tan, Xungang1,2 ; Li, Jun 1,2; Zhang, Peijun 1,2; You, Feng 1,2
	2017-08-30
发表期刊	GENE
卷号	626 页码:1-8
文章类型	Article
摘要	Steroidogenic acute regulatory protein 2 (StAR2) is a key protein in transporting cholesterol from the outer mitochondria membrane to the inner mitochondria membrane for sex steroid synthesis. In this study, two StAR2 gene isoforms, StAR2a and StAR2b, were isolated from the olive flounder Paralichthys olivaceus gonads. Semi quantitative RT-PCR results indicated that their expression levels were higher in testis than those in ovary. StAR2a was mainly expressed in the thecal cells and ooplasm of ovary, and Leydig cells and spermatid of testis according to the results of in situ hybridization. The quantitative real-time PCR results showed that the expressions of StAR2a and StAR2b were high in undifferentiation gonads and differentiating testis, and then decreased in differentiated testis in the high temperature (28 degrees C) and exogenous testosterone treatment groups. While, in the exogenous 17 beta-estradiol treatment group, both genes' expression levels were high in differentiating ovary, and then significantly decreased in differentiated ovary (P < 0.05). StAR2a and StAR2b expression levels were significantly down-regulated in the cultured testis cells treated with the 75 and 150 mu M cAMP, but significantly up-regulated in the cultured testis cells treated with the 300 mu M cAMP (P < 0.05). Moreover, their expression levels were significantly up-regulated by transfecting the cultured testis cells with pcDNA3.1-NR5a2 and pcDNA3.1-NR0b1 (P < 0.05). Above study showed that expression of StAR2 was regulated by cAMP and the transcription factors, NR5a2 and NR0b1, indicating that StAR2 may have functions in flounder gonadal differentiation and maintenance.
关键词	Paralichthys Olivaceus Star2 Cloning Expression Gonadal Differentiation Camp Nr5a2/nr0b1
DOI	10.1016/j.gene.2017.05.008
收录类别	SCI
语种	英语
WOS记录号	WOS:000407404600001
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/143101
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liang, Dongdong,Fan, Zhaofei,Weng, Shenda,et al. Characterization and expression of StAR2a and StAR2b in the olive flounder Paralichthys olivaceus[J]. GENE,2017,626:1-8.
APA	Liang, Dongdong.,Fan, Zhaofei.,Weng, Shenda.,Jiao, Shuang.,Wu, Zhihao.,...&You, Feng.(2017).Characterization and expression of StAR2a and StAR2b in the olive flounder Paralichthys olivaceus.GENE,626,1-8.
MLA	Liang, Dongdong,et al."Characterization and expression of StAR2a and StAR2b in the olive flounder Paralichthys olivaceus".GENE 626(2017):1-8.

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