IOCAS-IR  > 实验海洋生物学重点实验室
Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining
Chi, Heng1,4; Wen, Li-lian1,3; Sui, Zhi-Hai1,3; Sun, Qing-Lei1,2; Sun, Li1,2,4
2017-12-01
发表期刊TISSUE & CELL
卷号49期号:6页码:751-755
文章类型Article
摘要Cytomorphological and cytochemical staining are important methods for the identification of cell types, in particular in fish which often lack biological tools such as specific antibodies. Myeloperoxidase (MPO) is usually used as an intracellular marker of neutrophil accumulation in tissues and a marker of neutrophil activity in plasma. In this study, we reported a potassium iodide and oxidized pyronine Y (KI-PyY) staining method for rapid and highly sensitive detection of MPO-positive cells in turbot blood, peritoneum, and tissues. MPO-positive cells, which mostly represented neutrophils, were stained brown and clearly distinguished from other cells, such as lymphocytes, monocytes, and macrophages, which were stained pink. Following bacterial stimulation, the proportions of neutrophils were 27.49% and 38.05% in peripheral blood leukocytes and peritoneum, respectively, judging by the stained MPO. Kidney granulocytes contained abundant MPO-positive cells which were probably immature neutrophils with low expression of MPO. It is noteworthy that MPO-positive cells were detected in the tissue sections of kidney, spleen, and gut, with distribution profiles specific to each tissue. However, the cell morphology was not distinct in the stained tissue sections. These results indicate that the KI-PyY staining method is highly sensitive, applicable to different types of samples, and will be useful for the study of neutrophils in different compartments of fish.
关键词Cytochemical Identification Ki-pyy Turbot Mpo-positive Granulocytes
DOI10.1016/j.tice.2017.10.008
收录类别SCI
语种英语
WOS记录号WOS:000417042700015
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/143292
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位实验海洋生物学重点实验室;  中国科学院海洋研究所
推荐引用方式
GB/T 7714
Chi, Heng,Wen, Li-lian,Sui, Zhi-Hai,et al. Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining[J]. TISSUE & CELL,2017,49(6):751-755.
APA Chi, Heng,Wen, Li-lian,Sui, Zhi-Hai,Sun, Qing-Lei,&Sun, Li.(2017).Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining.TISSUE & CELL,49(6),751-755.
MLA Chi, Heng,et al."Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining".TISSUE & CELL 49.6(2017):751-755.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Cytochemical identif(834KB)期刊论文出版稿限制开放CC BY-NC-SA浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Chi, Heng]的文章
[Wen, Li-lian]的文章
[Sui, Zhi-Hai]的文章
百度学术
百度学术中相似的文章
[Chi, Heng]的文章
[Wen, Li-lian]的文章
[Sui, Zhi-Hai]的文章
必应学术
必应学术中相似的文章
[Chi, Heng]的文章
[Wen, Li-lian]的文章
[Sui, Zhi-Hai]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。