Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Gill symbionts of the cold-seep mussel Bathymodiolus platifrons: Composition, environmental dependency and immune control | |
Yu, Jiajia1,3 ; Wang, Minxiao1,2; Liu, Baozhong1,2; Yue, Xin1; Li, Chaolun1,2
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| 2019-03-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 86页码:246-252 |
| 通讯作者 | Yue, Xin(yuexin@qdio.ac.cn) ; Li, Chaolun(lcl@qdio.ac.cn) |
| 摘要 | Deep-sea Bathymodiolus mussels depend on the organic carbon supplied by symbionts inside their gills. In this study, optimized methods of quantitative real-time PCR and fluorescence in situ hybridization targeted to both mRNA and 16S rRNA were used to investigate the gill symbionts of the cold-seep mussel Bathymodiolus platifrons, including species composition, environmental dependency and immune control by the host. Our results showed that methanotrophs were the major symbiotic bacteria in the gills of B. platifrons, while thiotrophs were scarce. In the mussels freshly collected from the deep sea, methanotrophs were housed in bacteriocytes in a unique circular pattern, and a lysosome-related gene (VAMP) encoding a vesicle-associated membrane protein was expressed at a high level and presented exactly where the methanotrophs occurred. After the mussels were reared for three months in aquaria without methane supply, the abundance of methanotrophs decreased significantly and their circle-shaped distribution pattern disappeared; in addition, the expression of VAMP decreased significantly. These results suggest that the symbiosis between B. platifrons and methanotrophs is influenced by the environment and that the lysosomal system plays an important immune role in controlling the abundance of endosymbionts in host. This study provides a reliable method for investigating symbionts in deep-sea mussels and enriches the knowledge about symbionts in B. platifrons. |
| 关键词 | Bathymodiolus platifrons Methanotrophs FISH qRT-PCR Lysosomal system |
| DOI | 10.1016/j.fsi.2018.11.041 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Scientific and Technological Innovation Project from Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Youth Foundation Project[YQ2018NO07] ; International Partnership for Innovative Team Program from the Chinese Academy of Sciences[20140491526] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11030202] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11030202] ; International Partnership for Innovative Team Program from the Chinese Academy of Sciences[20140491526] ; Youth Foundation Project[YQ2018NO07] ; Scientific and Technological Innovation Project from Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000459528400029 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/155575 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Yue, Xin; Li, Chaolun |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Yu, Jiajia,Wang, Minxiao,Liu, Baozhong,et al. Gill symbionts of the cold-seep mussel Bathymodiolus platifrons: Composition, environmental dependency and immune control[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:246-252. |
| APA | Yu, Jiajia,Wang, Minxiao,Liu, Baozhong,Yue, Xin,&Li, Chaolun.(2019).Gill symbionts of the cold-seep mussel Bathymodiolus platifrons: Composition, environmental dependency and immune control.FISH & SHELLFISH IMMUNOLOGY,86,246-252. |
| MLA | Yu, Jiajia,et al."Gill symbionts of the cold-seep mussel Bathymodiolus platifrons: Composition, environmental dependency and immune control".FISH & SHELLFISH IMMUNOLOGY 86(2019):246-252. |
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