Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance

doi:10.1016/j.scienta.2018.08.058

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	Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance
	Jia, Dongjie 1; Fan, Lianmei 1; Shen, Junling 1; Qin, Song 2; Li, Fuchao 2; Yuan, Yongbing 1
	2019-01-03
发表期刊	SCIENTIA HORTICULTURAE
ISSN	0304-4238
卷号	243 页码:428-433
通讯作者	Yuan, Yongbing(yyb@qau.edu.cn)
摘要	Apples may suffer sunburn during growing season because of photooxidation damage. Astaxanthin have a stronger antioxidant activity against photooxidation compared to its precursors zeaxanthin and beta-carotene. Here, we transferred beta-carotene ketolase (bkt) and beta-carotene hydroxylase (crtR-B), two genes encoding key enzymes for astaxanthin biosynthesis, into "Brookfield Gala" apple plants using pCAMBIA1302-bkt-crtR-B via transformation mediated by Agrobacterium tumefaciens EHA105. We aimed to confer a stronger antioxidant ability against sunburn to apple plants by regulating metabolic pathways of carotenoids. RT-PCR and qPCR analysis confirmed that bkt and crtR-B genes were expressed simultaneously in transgenic plants. Southern blotting analysis confirmed that the transgene was stably integrated and determined transgene copy number. Transgenic plants synthesized novel ketocarotenoids, resulting in an accumulation of astaxanthin up to 12.06 mu g/g and of canthaxanthin up to 6.38 mu g/g per leaf fresh weight. The light antioxidant ability of transgenic plantlets was stronger than that of non-transgenic ones in high light conditions. We speculate that the transgenic apples accumulating astaxanthin are resistant to photooxidation and can therefore prevent sunburn.
关键词	Malus domestica Borkh Astaxanthin beta-Carotene ketolase beta-Carotene hydroxylase Photoprotection
DOI	10.1016/j.scienta.2018.08.058
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2016YFD0400100] ; project of key agricultural technique research of Shandong Province ; [NSFC30471200] ; National Key Research and Development Program of China[2016YFD0400100] ; project of key agricultural technique research of Shandong Province ; [NSFC30471200]
WOS研究方向	Agriculture
WOS类目	Horticulture
WOS记录号	WOS:000450381800053
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/156385
专题	中国科学院海洋研究所
通讯作者	Yuan, Yongbing
作者单位	1.Qingdao Agr Univ, Coll Hort, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Jia, Dongjie,Fan, Lianmei,Shen, Junling,et al. Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance[J]. SCIENTIA HORTICULTURAE,2019,243:428-433.
APA	Jia, Dongjie,Fan, Lianmei,Shen, Junling,Qin, Song,Li, Fuchao,&Yuan, Yongbing.(2019).Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance.SCIENTIA HORTICULTURAE,243,428-433.
MLA	Jia, Dongjie,et al."Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance".SCIENTIA HORTICULTURAE 243(2019):428-433.

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