Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation | |
Wang, Xueying1,2 ; Liu, Qinghua1,2; Xu, Shihong1,2; Xiao, Yongshuang1,2; Wang, Yanfeng1,2; Feng, Chengcheng1,2; Xue, Rui1,2,3; Zhao, Haixia1,2,3; Song, Zongcheng4; Li, Jun1,2
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| 2018-10-25 | |
| 发表期刊 | SCIENTIFIC REPORTS
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| ISSN | 2045-2322 |
| 卷号 | 8页码:12 |
| 通讯作者 | Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| 摘要 | Spermatogenesis is a dynamic developmental process in which spermatogonial stem cells proliferate, differentiate and mature into functional spermatozoa. These processes require an accurate gene regulation network. Here, we investigated the dynamic changes that occur during spermatogenesis through a combination of histological and transcriptome analyses of different developmental stages of the testis. We constructed 18 testis transcriptome libraries, and the average length, N50, and GC content of the unigenes were 1,795 bp; 3,240 bp and 49.25%, respectively. Differentially expressed genes (DEGs) that were related to germ cell proliferation and maturation, such as NANOS3, RARs, KIFs, steroid hormone synthesis-related genes and receptor genes, were identified between pairs of testis at different developmental stages. Gene ontology annotation and pathway analyses were conducted on DEGs with specific expression patterns involved in the regulation of spermatogenesis. Nine important pathways such as steroid hormone biosynthesis related to spermatogenesis were identified. A total of 21 modules that ranged from 49 to 7,448 genes were designed by a weighted gene co-expression network analysis. Furthermore, a total of 83 candidate miRNA were identified by computational methods. Our study provides the first transcriptomic evidence for differences in gene expression between different developmental stages of spermatogenesis in turbot (Scophthalmus maximus). |
| DOI | 10.1038/s41598-018-34149-5 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; National Natural Science Foundation of China[31372514] ; China Agriculture Research System[CARS-47] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] ; Youth Innovation Promotion Association CAS ; Scientific and Technological Innovation Project ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; National Natural Science Foundation of China[31372514] ; China Agriculture Research System[CARS-47] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Chinese Academy of science and technology service network planning[KFJ-EW-STS-060] ; Youth Innovation Promotion Association CAS ; Scientific and Technological Innovation Project |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000448271600024 |
| 出版者 | NATURE PUBLISHING GROUP |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/156568 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Qinghua; Li, Jun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Xueying,Liu, Qinghua,Xu, Shihong,et al. Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation[J]. SCIENTIFIC REPORTS,2018,8:12. |
| APA | Wang, Xueying.,Liu, Qinghua.,Xu, Shihong.,Xiao, Yongshuang.,Wang, Yanfeng.,...&Li, Jun.(2018).Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation.SCIENTIFIC REPORTS,8,12. |
| MLA | Wang, Xueying,et al."Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation".SCIENTIFIC REPORTS 8(2018):12. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Transcriptome Dynami（2610KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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