Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1 | |
Wang, M.1; Wang, B.1; Liu, M.1; Jiang, K.1; Wang, L.1,2 | |
2017 | |
发表期刊 | ISJ-INVERTEBRATE SURVIVAL JOURNAL |
ISSN | 1824-307X |
卷号 | 14页码:453-463 |
通讯作者 | Wang, L.(wanglei@qdio.ac.cn) |
摘要 | Macrophage migration inhibitory factor (MIF) was an ancient cytokine and involved in innate immunity of vertebrates and invertebrates. In the present study, a novel MIF homologue (designated as LvMIF2) has been cloned from the Pacific white shrimp Litopenaeus vannamei via rapid amplification of cDNA ends (RACE) technique. The full-length cDNA sequence of LvMIF2 was 555 bp and contained a 97 bp 5' untranslated region (UTR) and a 3' UTR of 110 bp, and an open reading frame (ORF) of 348 bp which coded 115 amino acids. Quantitative realtime PCR (qRT-PCR) analysis indicated that LvMIF2 was constitutively expressed in all the tested tissues, including eyestalk, gill, gonad, heart, hemocytes, hepatopancreas, intestine, muscle, nerve and stomach, with the highest mRNA expression level in hemocytes and hepatopancreas. After Vibrio parahaemolyticus or white spot syndrome virus (WSSV) challenge, the mRNA expression levels of LvMIF2 in hemocytes and hepatopancreas were all sharply upregulated. Comparative sequence and transcription analysis between the previously identified LvMIF1 and LvMIF2 revealed that both LvMIF1 and LvMIF2 might play crucial and functionally differentiated roles in shrimp innate immune responses to bacterial and viral stimulation. |
关键词 | Litopenaeus vannamei macrophage migration inhibitory factor innate immunity |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Major Projects of Shandong Province[2015ZDZX05002] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Major Projects of Shandong Province[2015ZDZX05002] |
WOS研究方向 | Immunology ; Zoology |
WOS类目 | Immunology ; Zoology |
WOS记录号 | WOS:000416558800003 |
出版者 | INVERTEBRATE SURVIVAL JOURNAL |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/157276 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Wang, L. |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China |
第一作者单位 | 实验海洋生物学重点实验室 |
通讯作者单位 | 实验海洋生物学重点实验室 |
推荐引用方式 GB/T 7714 | Wang, M.,Wang, B.,Liu, M.,et al. A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2017,14:453-463. |
APA | Wang, M.,Wang, B.,Liu, M.,Jiang, K.,&Wang, L..(2017).A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1.ISJ-INVERTEBRATE SURVIVAL JOURNAL,14,453-463. |
MLA | Wang, M.,et al."A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1".ISJ-INVERTEBRATE SURVIVAL JOURNAL 14(2017):453-463. |
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