Characterization and transcriptional analysis of one carbonic anhydrase gene in the green-tide-forming alga Ulva prolifera (Ulvophyceae, Chlorophyta)

doi:10.1111/pre.12402

	Characterization and transcriptional analysis of one carbonic anhydrase gene in the green-tide-forming alga Ulva prolifera (Ulvophyceae, Chlorophyta)
	Wang, Yu 1,2,3; Liu, Feng 2,3,4; Wang, Mengqiang2,3,4 ; Moejes, Fiona W.5; Bi, Yuping 1,6
	2019-09-06
发表期刊	PHYCOLOGICAL RESEARCH
ISSN	1322-0829
页码	8
通讯作者	Liu, Feng(liufeng@qdio.ac.cn) ; Bi, Yuping(yupingbi@vip.sina.com)
摘要	Ulva prolifera is a green-tide-forming macroalga dominating the green tides in the Yellow Sea from 2007 to 2019. The exponential growth of U. prolifera, and the correlating accumulation of biomass, rely heavily on its carbon fixation capability. Despite the importance of carbon fixation in algal growth, to date none of the genes involved in carbon fixation have undergone molecular characterization in U. prolifera. This study used Rapid Amplification of cDNA Ends (RACE) to characterize one full-length carbonic anhydrase (CA) gene of U. prolifera (Up alpha CA1). An activity assay showed that Up alpha CA1 was an active CA. Real-time quantitative PCR results showed that temperature, irradiance, salinity and pH could influence the transcriptional level of Up alpha CA1, indicating that Up alpha CA1 was sensitive to varying environmental factors. The results of this study provided further insights into our understanding of the influence of environmental factors on carbon fixation in U. prolifera and its subsequent role in the formation of green tides.
关键词	Chlorophyta environmental factor green tide transcriptional level Yellow Sea
DOI	10.1111/pre.12402
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050302] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050403] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0504] ; National Natural Science Foundation of China[41876165] ; Key Research Program of Frontier Sciences, Chinese Academy of Sciences[QYZDB-SSW-DQC023] ; Scientific and Technological Innovation Project - Pilot National Laboratory for Marine Science and Technology (Qingdao)[2016ASKJ02] ; Youth Innovation Promotion Association, Chinese Academy of Sciences[2015164] ; Open Research Fund of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, S.O.A.[MATHAB201701] ; Earmarked Fund for Modern Agro-industry Technology Research System in Shandong Province of China[SDAIT-26-09] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050302] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050403] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0504] ; National Natural Science Foundation of China[41876165] ; Key Research Program of Frontier Sciences, Chinese Academy of Sciences[QYZDB-SSW-DQC023] ; Scientific and Technological Innovation Project - Pilot National Laboratory for Marine Science and Technology (Qingdao)[2016ASKJ02] ; Youth Innovation Promotion Association, Chinese Academy of Sciences[2015164] ; Open Research Fund of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, S.O.A.[MATHAB201701] ; Earmarked Fund for Modern Agro-industry Technology Research System in Shandong Province of China[SDAIT-26-09]
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:000486695800001
出版者	WILEY
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/162975
专题	海洋生态与环境科学重点实验室
通讯作者	Liu, Feng; Bi, Yuping
作者单位	1.Shandong Univ, Sch Life Sci, Jinan, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Marine Ecol & Environm Sci Lab, Qingdao, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Shandong, Peoples R China 5.Bantry Marine Res Stn Ltd, Bantry, Ireland 6.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Yu,Liu, Feng,Wang, Mengqiang,et al. Characterization and transcriptional analysis of one carbonic anhydrase gene in the green-tide-forming alga Ulva prolifera (Ulvophyceae, Chlorophyta)[J]. PHYCOLOGICAL RESEARCH,2019:8.
APA	Wang, Yu,Liu, Feng,Wang, Mengqiang,Moejes, Fiona W.,&Bi, Yuping.(2019).Characterization and transcriptional analysis of one carbonic anhydrase gene in the green-tide-forming alga Ulva prolifera (Ulvophyceae, Chlorophyta).PHYCOLOGICAL RESEARCH,8.
MLA	Wang, Yu,et al."Characterization and transcriptional analysis of one carbonic anhydrase gene in the green-tide-forming alga Ulva prolifera (Ulvophyceae, Chlorophyta)".PHYCOLOGICAL RESEARCH (2019):8.

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