Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus

doi:10.3389/fmars.2019.00637

IOCAS-IR > 实验海洋生物学重点实验室

	Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus
	Wu, Zhihao 1,2; Wang, Lijuan1,2 ; Lu, Yunliang 3; Zhu, Xiangping 3; Yue, Xinlu 4; You, Feng 1,2
	2019-10-16
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	6 页码:12
通讯作者	You, Feng(youfeng@qdio.ac.cn)
摘要	Artificial tetraploid induction is one of the important techniques of fish chromosome manipulation, and it is the first step for triploid breeding. There are a few reports to artificial induction of tetraploid in marine fish. The induction and survival rates were usually low. We firstly optimized the tetraploid induction conditions in turbot Scophthalmus maximus, one of the most important maricultural fish in China and Europe. For the initiate time of treatment, which is the most important factor in tetraploid induction, the first cleavage index (FCI) was used to reduce the influences of genetic origin and environment factors. Overall, the optimal initiation time for pressure shock was 15 min before the first cleavage at 14.8-15.5 degrees C. The optimal treatment pressure and treatment duration were 67.5 MPa and 6 min. The regression equation prediction model was: The optimal initiation time = 0.982 FCI - 12.182 or the optimal initiation time = 0.85 FCI. Then two tetraploid induction (4n1 and 4n2) populations were obtained under the optimal conditions with diploid controls (2n1 and 2n2). The induction rates in tetraploid induction (4n) populations at hatched larvae stage could reach 100%. The genetic structure of these two 4n populations was also studied. Two to four alleles in each locus were detected in diploid (2n) and 4n populations, respectively. Private alleles were only appeared at locus Sma-USC21, with two alleles lost in 4n populations. Eleven and fourteen loci in 2n and 4n populations respectively showed a negative genetic deviation index. 3D-FCA analysis showed that the two 2n and two 4n populations have obvious differences. The numbers of locus deviating from Hardy-Weinberg equilibrium in 2n1, 4n1, 2n2, and 4n2 populations were 6, 9, 12, and 7, respectively. Overall, 12 loci in either 2n or 4n population deviated from Hardy-Weinberg equilibrium. Tetraploid induction population showed lower heterozygosity and higher heterozygote deletion.
关键词	turbot Scophthalmus maximus tetraploid artificial induction first cleavage index genetic diversity
DOI	10.3389/fmars.2019.00637
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31502156] ; National Natural Science Foundation of China[41276171] ; Key Research and Development Plan of Shandong Province[2017CXGC0101] ; National Key R&D Program of China[2018YFD0901202] ; National Natural Science Foundation of China[31502156] ; National Natural Science Foundation of China[41276171] ; Key Research and Development Plan of Shandong Province[2017CXGC0101] ; National Key R&D Program of China[2018YFD0901202]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000490444800001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/163254
专题	实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Qingdao Agr Univ Qingdao, Marine Sci & Engineer Coll, Qingdao, Shandong, Peoples R China 4.Shenghang Aquat Sci & Technol Co Ltd, Weihai, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wu, Zhihao,Wang, Lijuan,Lu, Yunliang,et al. Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus[J]. FRONTIERS IN MARINE SCIENCE,2019,6:12.
APA	Wu, Zhihao,Wang, Lijuan,Lu, Yunliang,Zhu, Xiangping,Yue, Xinlu,&You, Feng.(2019).Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus.FRONTIERS IN MARINE SCIENCE,6,12.
MLA	Wu, Zhihao,et al."Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus".FRONTIERS IN MARINE SCIENCE 6(2019):12.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
Wu-2019-Artificial-i（2002KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览
Artificial Induction（2002KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览