Microbial genetic diversity and ciliate community structure along an environmental gradient in coastal soil

doi:10.1016/j.ejop.2013.01.002

	Microbial genetic diversity and ciliate community structure along an environmental gradient in coastal soil
	Zhao, Feng 1,2; Xu, Kuidong1 ; Xu, KD
	2013-11-01
发表期刊	EUROPEAN JOURNAL OF PROTISTOLOGY
ISSN	0932-4739
卷号	49 期号:4 页码:516-525
文章类型	Article
摘要	We investigated the microbial genetic diversity and ciliate community in coastal soil from five sites with an environmental gradient using denaturing gradient gel electrophoresis (DGGE), gene sequencing and the Ludox-QPS method. The analyses of both the 16S ribosomal RNA (rRNA) gene and 18S rRNA gene DGGE resulted in equal or even a higher number of bands found in the samples taken from the high-salinity sites IV and V than in those taken from the low-to-moderate-salinity sites I-III. Cluster analysis of both DGGE profiles classified the five sites into three main groups (sites I, II and III and IV and V), which corresponded well to the analysis of environmental factors. There were 13 species observed at site I, three species at site II and nine species at site III, while no active ciliates were observed at the high-salinity sites IV and V. By contrast, the ciliate-specific DGGE revealed a higher number of bands in the samples taken from the high-salinity soil. Furthermore, gene sequencing suggested that the ciliates in the high-salinity soil comprised forms originating not only from soil but also from marine environments. The data indicate that saline soil may maintain a high diversity of ciliates and soil salinity might be the most influential factor regulating the community structure of ciliates. (C) 2013 Elsevier GmbH. All rights reserved.; We investigated the microbial genetic diversity and ciliate community in coastal soil from five sites with an environmental gradient using denaturing gradient gel electrophoresis (DGGE), gene sequencing and the Ludox-QPS method. The analyses of both the 16S ribosomal RNA (rRNA) gene and 18S rRNA gene DGGE resulted in equal or even a higher number of bands found in the samples taken from the high-salinity sites IV and V than in those taken from the low-to-moderate-salinity sites I-III. Cluster analysis of both DGGE profiles classified the five sites into three main groups (sites I, II and III and IV and V), which corresponded well to the analysis of environmental factors. There were 13 species observed at site I, three species at site II and nine species at site III, while no active ciliates were observed at the high-salinity sites IV and V. By contrast, the ciliate-specific DGGE revealed a higher number of bands in the samples taken from the high-salinity soil. Furthermore, gene sequencing suggested that the ciliates in the high-salinity soil comprised forms originating not only from soil but also from marine environments. The data indicate that saline soil may maintain a high diversity of ciliates and soil salinity might be the most influential factor regulating the community structure of ciliates. (C) 2013 Elsevier GmbH. All rights reserved.
关键词	Ciliate Community Coastal Soil Molecular Diversity Salinisation
学科领域	Microbiology
DOI	10.1016/j.ejop.2013.01.002
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000326360200003
WOS关键词	16S RIBOSOMAL-RNA ; SALINITY GRADIENT ; MOLECULAR TECHNIQUES ; BACTERIAL ; CHINA ; DGGE ; ASSEMBLAGES ; PATTERNS ; PROTOZOA ; RIVER
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16336
专题	海洋生物分类与系统演化实验室
通讯作者	Xu, KD
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhao, Feng,Xu, Kuidong,Xu, KD. Microbial genetic diversity and ciliate community structure along an environmental gradient in coastal soil[J]. EUROPEAN JOURNAL OF PROTISTOLOGY,2013,49(4):516-525.
APA	Zhao, Feng,Xu, Kuidong,&Xu, KD.(2013).Microbial genetic diversity and ciliate community structure along an environmental gradient in coastal soil.EUROPEAN JOURNAL OF PROTISTOLOGY,49(4),516-525.
MLA	Zhao, Feng,et al."Microbial genetic diversity and ciliate community structure along an environmental gradient in coastal soil".EUROPEAN JOURNAL OF PROTISTOLOGY 49.4(2013):516-525.

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