Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53

doi:10.1016/j.dci.2019.103531

IOCAS-IR > 实验海洋生物学重点实验室

	Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53
	Guan, Xiao-lu 1,2; Zhang, Bao-cun 3,4; Sun, Li 1,2
	2020-02-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	103 页码:8
通讯作者	Sun, Li(Isun@qdio.ac.cn)
摘要	MicroRNAs (miRNAs) are post-transcriptional regulators that play vital roles in diverse physiological processes including immunity. In this study, we investigated the regulatory mechanism and function of a novel Japanese flounder (Paralichthys olivaceus) miRNA, pol-miR-3p-2. pol-miR-3p-2 was responsive in expression to the infection of the bacterial pathogen Edwardsiella tarda. pol-miR-3p-2 negatively regulated the expression of p53 through interaction with the 3'UTR of p53. Overexpression of pol-miR-3p-2 promoted autophagy, resulting in augmented production of LC3-II, while knockdown of p53 increased the level of beclin, a key factor of autophagy. In vivo and in vitro studies showed that E. tarda infection induced autophagy in flounder, and pol-miR-3p-2 inhibited the infectivity of E. tarda. Together these results indicate that pol-miR-3p-2 regulates autophagy through the target gene p53, thus revealing a regulatory link between p53 and autophagy in teleost, and that pol-miR-3p-2 plays an important role in the immune defense against E. tarda.
关键词	miRNA Paralichthys olivaceus Autophagy Bacterial infection Immune defense
DOI	10.1016/j.dci.2019.103531
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31730100] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province ; National Natural Science Foundation of China[31730100] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000500050700027
出版者	ELSEVIER SCI LTD
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/163748
专题	实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Aarhus Univ, Dept Biomed, Aarhus, Denmark 4.Aarhus Univ, Aarhus Res Ctr Innate Immun, Aarhus, Denmark
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Guan, Xiao-lu,Zhang, Bao-cun,Sun, Li. Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,103:8.
APA	Guan, Xiao-lu,Zhang, Bao-cun,&Sun, Li.(2020).Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,103,8.
MLA	Guan, Xiao-lu,et al."Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 103(2020):8.

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