Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine | |
Yang, Yue1,2,3; Xing, Ronge1,2![]() ![]() ![]() | |
2020-02-01 | |
发表期刊 | CARBOHYDRATE POLYMERS
![]() |
ISSN | 0144-8617 |
卷号 | 229页码:9 |
通讯作者 | Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn) |
摘要 | Chitosan (CS) and its water-soluble derivatives, hydroxypropyltrimethyl ammonium chloride chitosan (HACC) and sulfated chitosan (SCS), were used as adjuvants of inactivated Newcastle disease (ND) vaccine. First, NDV-loaded and blank CS, HACC/CS and SCS nanoparticles were prepared. The particle sizes were respectively 343.43 +/- 4.12, 320.03 +/- 0.84, 156.2 +/- 9.29 nm and the zeta potentials were respectively +19.67 +/- 0.58, +18.3 +/- 0.5, -17.8 +/- 2.65 mV under the optimal conditions. Then chickens were immunized with nanoparticles or commercial inactivated oil emulsion vaccine. After immunization, the humoral immunity levels of the chickens were evaluated. The cellular immunity levels were determined by the quantification of cytokines, lymphocyte proliferation assay, the percentages of CD4(+) and CD8(+) T lymphocytes. Finally, the chickens were challenged with highly virulent virus. The results demonstrated that the humoral immunity levels in NDV-loaded CS and HACC/CS nanoparticles groups were lower than commercial vaccine but the cellular immunity levels are better. Moreover, the prevention effects of NDV-loaded CS and HACC/CS nanoparticles against highly virulent NDV are comparable to commercial vaccine. Our study provides the basis of developing HACC and CS as effective vaccine adjuvants. |
关键词 | Newcastle disease virus Adjuvant Nanoparticle Chitosan Hydroxypropyltrimethyl ammonium chloride chitosan Sulfated chitosan |
DOI | 10.1016/j.carbpol.2019.115423 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Key R&D Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2019GHY112015] ; Key Research and Development Program of Shandong Province[2017YYSP018] ; Special projects of Foshan science and technology innovation team[2017IT100054] ; National Key R&D Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2019GHY112015] ; Key Research and Development Program of Shandong Province[2017YYSP018] ; Special projects of Foshan science and technology innovation team[2017IT100054] |
WOS研究方向 | Chemistry ; Polymer Science |
WOS类目 | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
WOS记录号 | WOS:000501796600026 |
出版者 | ELSEVIER SCI LTD |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/164170 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Xing, Ronge; Li, Pengcheng |
作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Ctr Ocean Mega Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Yang, Yue,Xing, Ronge,Liu, Song,et al. Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine[J]. CARBOHYDRATE POLYMERS,2020,229:9. |
APA | Yang, Yue.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2020).Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine.CARBOHYDRATE POLYMERS,229,9. |
MLA | Yang, Yue,et al."Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine".CARBOHYDRATE POLYMERS 229(2020):9. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Chitosan, hydroxypro(1152KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论