A four-CRD C-type lectin from Chlamys farreri mediating nonself-recognition with broader spectrum and opsonization

doi:10.1016/j.dci.2012.12.002

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	A four-CRD C-type lectin from Chlamys farreri mediating nonself-recognition with broader spectrum and opsonization
	Huang, Mengmeng1,2 ; Wang, Lingling 1; Yang, Jialong 1,2; Zhang, Huan 1; Wang, Leilei 1,2; Song, Linsheng 1; Zhang, H
	2013-04-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	39 期号:4 页码:363-369
文章类型	Article
摘要	C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins consisting of at least one carbohydrate-recognition domain (CRD), which participate in nonself-recognition and clearance of invaders. In invertebrate, some multidomain C-type lectins have been identified, but their relative functions and binding mechanism are still meager. In the present study, A C-type lectin (CfLec-4) with four CRDs from Chlamys farreri was selected to investigate its possible function in innate immunity. The mRNA expression of CfLec-4 in hemocytes was significantly up-regulated (P < 0.01) after the stimulations of beta-glucan, LPS or PGN, and reached the highest expression level at 3, 6, 12 h post-stimulation, which was 27.9-, 22.6- or 47.9-fold of that in blank group, respectively. Immunohistochemistry assay with polyclonal antibody specific for CfLec-4 revealed that the endogenous CfLec-4 was mainly located in the hepatopancreas, kidney and gonad of the scallops. The recombinant CfLec-4 (rCflec-4) could bind LPS, PGN, glucan and mannose in vitro, but could not bind LTA. Furthermore, rCflec-4 displayed a broader bacteria binding spectrum towards Gram-positive bacteria Staphylococcus aureus and Micrococcus luteus as well as Gram-negative bacteria Escherichia coil, Vibrio anguillarum and fungi Pichia pastoris. Meanwhile, rCfLec-4 could significantly (P < 0.01) enhance the phagocytosis of hemocytes in vitro. The results clearly suggested that four-CRD containing CfLec-4 not only served as PRR with wider recognition spectrum, but also functioned as an opsonin participating in the clearance of invaders in scallops. It could be inferred that the diversity and complexity of CRDs in C-type lectins endowed these receptors with comprehensive recognition spectrum and multiple immune functions against complex living environment. (C) 2012 Elsevier Ltd. All rights reserved.; C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins consisting of at least one carbohydrate-recognition domain (CRD), which participate in nonself-recognition and clearance of invaders. In invertebrate, some multidomain C-type lectins have been identified, but their relative functions and binding mechanism are still meager. In the present study, A C-type lectin (CfLec-4) with four CRDs from Chlamys farreri was selected to investigate its possible function in innate immunity. The mRNA expression of CfLec-4 in hemocytes was significantly up-regulated (P < 0.01) after the stimulations of beta-glucan, LPS or PGN, and reached the highest expression level at 3, 6, 12 h post-stimulation, which was 27.9-, 22.6- or 47.9-fold of that in blank group, respectively. Immunohistochemistry assay with polyclonal antibody specific for CfLec-4 revealed that the endogenous CfLec-4 was mainly located in the hepatopancreas, kidney and gonad of the scallops. The recombinant CfLec-4 (rCflec-4) could bind LPS, PGN, glucan and mannose in vitro, but could not bind LTA. Furthermore, rCflec-4 displayed a broader bacteria binding spectrum towards Gram-positive bacteria Staphylococcus aureus and Micrococcus luteus as well as Gram-negative bacteria Escherichia coil, Vibrio anguillarum and fungi Pichia pastoris. Meanwhile, rCfLec-4 could significantly (P < 0.01) enhance the phagocytosis of hemocytes in vitro. The results clearly suggested that four-CRD containing CfLec-4 not only served as PRR with wider recognition spectrum, but also functioned as an opsonin participating in the clearance of invaders in scallops. It could be inferred that the diversity and complexity of CRDs in C-type lectins endowed these receptors with comprehensive recognition spectrum and multiple immune functions against complex living environment. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Chlamys Farreri C-type Lectin Innate Immunity Nonself-recognition Opsonization Microbe Binding Ability
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2012.12.002
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
WOS记录号	WOS:000317248900006
WOS关键词	PATTERN-RECOGNITION ; INNATE IMMUNITY ; BINDING LECTIN ; MANDUCA-SEXTA ; PATHOGEN RECOGNITION ; COMPLEMENT PATHWAY ; SELF-RECOGNITION ; BOMBYX-MORI ; PROTEIN ; LIPOPOLYSACCHARIDE
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：39[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16511
专题	实验海洋生物学重点实验室
通讯作者	Zhang, H
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Huang, Mengmeng,Wang, Lingling,Yang, Jialong,et al. A four-CRD C-type lectin from Chlamys farreri mediating nonself-recognition with broader spectrum and opsonization[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,39(4):363-369.
APA	Huang, Mengmeng.,Wang, Lingling.,Yang, Jialong.,Zhang, Huan.,Wang, Leilei.,...&Zhang, H.(2013).A four-CRD C-type lectin from Chlamys farreri mediating nonself-recognition with broader spectrum and opsonization.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,39(4),363-369.
MLA	Huang, Mengmeng,et al."A four-CRD C-type lectin from Chlamys farreri mediating nonself-recognition with broader spectrum and opsonization".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 39.4(2013):363-369.

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