Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
The trypsin-like serine protease domain of Paralichthys olivaceus complement factor I regulates complement activation and inhibits bacterial growth | |
Jia, Bei-bei1,2,3; Jin, Cheng-dong1,2,3; Li, Mo-fei1,2 | |
2020-02-01 | |
发表期刊 | FISH & SHELLFISH IMMUNOLOGY |
ISSN | 1050-4648 |
卷号 | 97页码:18-26 |
通讯作者 | Li, Mo-fei(murphy210@163.com) |
摘要 | In mammals, complement factor I (CFI) is a serine protease in serum and plays a pivotal role in the regulation of complement activation. In the presence of cofactor, CFI cleaves C3b to iC3b, and further degrades iC3b to C3c and C3d. In teleost, the function of CFI is poorly understood. In this study, we examined the immunological property of CFI from Japanese flounder (Paralichthys olivaceus) (PoCFI), a teleost species with important economic value. PoCFI is composed of 597 amino acid residues and possesses a trypsin-like serine protease (Tryp) domain. We found that PoCFI expressions occurred in nine different tissues and were upregulated by bacterial challenge. Recombinant PoCFI-Tryp (rPoCFI-Tryp) inhibited complement activation and degraded C3b in serum. rPoCFI-Tryp exhibited apparent binding capacities to a board-spectrum of bacteria and inhibited bacterial growth. These results provide the first evidence to indicate that CFI in teleost negatively regulates complement activation via degradation C3b, and probably plays a role in host immune defense against bacterial infection. |
关键词 | Factor I Complement Paralichthys olivaceus Antibacterial |
DOI | 10.1016/j.fsi.2019.12.019 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[31972831] ; National Natural Science Foundation of China[31602197] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2017249] ; Huiquan Young Scholar Program of Institute of Oceanology, CAS ; Taishan Scholar Program of Shandong Province |
WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS记录号 | WOS:000513983700003 |
出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/165512 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Li, Mo-fei |
作者单位 | 1.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Jia, Bei-bei,Jin, Cheng-dong,Li, Mo-fei. The trypsin-like serine protease domain of Paralichthys olivaceus complement factor I regulates complement activation and inhibits bacterial growth[J]. FISH & SHELLFISH IMMUNOLOGY,2020,97:18-26. |
APA | Jia, Bei-bei,Jin, Cheng-dong,&Li, Mo-fei.(2020).The trypsin-like serine protease domain of Paralichthys olivaceus complement factor I regulates complement activation and inhibits bacterial growth.FISH & SHELLFISH IMMUNOLOGY,97,18-26. |
MLA | Jia, Bei-bei,et al."The trypsin-like serine protease domain of Paralichthys olivaceus complement factor I regulates complement activation and inhibits bacterial growth".FISH & SHELLFISH IMMUNOLOGY 97(2020):18-26. |
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文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
The trypsin-like ser(10799KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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