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| Expression and function analysis of Rac1 homolog in Chinese shrimp Fenneropenaeus chinensis | |
| Chi, Yanhong1,2; Li, Fuhua1; Sun, Yumiao1; Wen, Rong1,2; Li, Shihao1; Li, FH | |
| 2013-09-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 35期号:3页码:927-932 |
| 文章类型 | Article |
| 摘要 | Rac1 is a ubiquitous GTP-binding protein that plays a crucial role in multiple cellular processes. In the present study, a Rac1 homolog (FcRac1) was cloned from the Chinese shrimp Fenneropenaeus chinensis. The open reading frame (ORF) of FcRac1 consists of 579 bp encoding 192 aa. The predicted molecular weight (MW) of the deduced amino acid sequence of FcRac1 was 21.46 kDa, and its theoretical pI was 8.62. Homology analysis showed that the amino acid sequence of Rac1 had high conservation among those from different species. Phylogenetic analysis showed that FcRac1 closely related to Rac1 proteins from other arthropods. FcRac1 showed the highest expression level in the hemocytes. In situ hybridization detection showed that it distributed in all types of hemocytes. Recombinant protein of FcRac1 showed apparent activity of GTPase. The transcription of FcRac1 in juvenile shrimp changed after bacteria or WSSV challenge. The present data suggests that FcRac1 might play important roles in the innate immunity of shrimp. (C) 2013 Elsevier Ltd. All rights reserved.; Rac1 is a ubiquitous GTP-binding protein that plays a crucial role in multiple cellular processes. In the present study, a Rac1 homolog (FcRac1) was cloned from the Chinese shrimp Fenneropenaeus chinensis. The open reading frame (ORF) of FcRac1 consists of 579 bp encoding 192 aa. The predicted molecular weight (MW) of the deduced amino acid sequence of FcRac1 was 21.46 kDa, and its theoretical pI was 8.62. Homology analysis showed that the amino acid sequence of Rac1 had high conservation among those from different species. Phylogenetic analysis showed that FcRac1 closely related to Rac1 proteins from other arthropods. FcRac1 showed the highest expression level in the hemocytes. In situ hybridization detection showed that it distributed in all types of hemocytes. Recombinant protein of FcRac1 showed apparent activity of GTPase. The transcription of FcRac1 in juvenile shrimp changed after bacteria or WSSV challenge. The present data suggests that FcRac1 might play important roles in the innate immunity of shrimp. (C) 2013 Elsevier Ltd. All rights reserved. |
| 关键词 | Rac1 Immunity Bacteria Wssv Fenneropenaeus Chinensis |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2013.07.006 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000324511700036 |
| WOS关键词 | GTP-BINDING PROTEINS ; RHO-GTPASES ; GENE-EXPRESSION ; KINASE PATHWAY ; IDENTIFICATION ; RECEPTOR ; LOCALIZATION ; CLONING ; FAMILY ; CDC42 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16568 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Li, FH |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Chi, Yanhong,Li, Fuhua,Sun, Yumiao,et al. Expression and function analysis of Rac1 homolog in Chinese shrimp Fenneropenaeus chinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):927-932. |
| APA | Chi, Yanhong,Li, Fuhua,Sun, Yumiao,Wen, Rong,Li, Shihao,&Li, FH.(2013).Expression and function analysis of Rac1 homolog in Chinese shrimp Fenneropenaeus chinensis.FISH & SHELLFISH IMMUNOLOGY,35(3),927-932. |
| MLA | Chi, Yanhong,et al."Expression and function analysis of Rac1 homolog in Chinese shrimp Fenneropenaeus chinensis".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):927-932. |
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| Expression and funct（978KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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