Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation

doi:10.1007/s00343-013-2164-5

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	Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation
	Chen Huaxin 1; Lin Hanzhi 1,2; Jiang Peng 1; Li Fuchao 1; Qin Song 3; Jiang, P
	2013-03-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	31 期号:2 页码:416-420
文章类型	Article
摘要	Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.; Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.
关键词	Chromatic Acclimation Electroporation Genetic Transformation Synechococcus Sp Cc9311
学科领域	Marine & Freshwater Biology ; Oceanography
DOI	10.1007/s00343-013-2164-5
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000316018300018
WOS关键词	CHROMATIC ADAPTATION ; MANIPULATION ; DIVERSITY ; MOTILITY ; STRAINS ; GENUS
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16579
专题	实验海洋生物学重点实验室
通讯作者	Jiang, P
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Chen Huaxin,Lin Hanzhi,Jiang Peng,et al. Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(2):416-420.
APA	Chen Huaxin,Lin Hanzhi,Jiang Peng,Li Fuchao,Qin Song,&Jiang, P.(2013).Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(2),416-420.
MLA	Chen Huaxin,et al."Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.2(2013):416-420.

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