Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis

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	Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis
	Li, Ying Xia 1,2; Wang, Guang Ce 2; Niu, Jian Feng 2; Gao, Zheng Quan ; Chen, Chang Sheng 3,4,5; Li, YX
	2013-02-01
发表期刊	INDIAN JOURNAL OF GEO-MARINE SCIENCES
ISSN	0379-5136
卷号	42 期号:1 页码:11-15
文章类型	Article
摘要	Thylakoid membranes were isolated and purified from gametophyte of Porphyra haitanesis by sucrose density gradient ultracentrifugation. After P. haitanesis gametophyte thylakoid membranes were solubilized with sodium dodecyl sulfate (SDS), the highly pure and active photosystem (PS) II complex was isolated and characterized by the room absorption and fluorescence emission spectra, DCIP (2, 6-Dichloroindophenol) reduction and oxygen- evolving rates. The PS II complex from P. haitanesis had the characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 urn) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Acquired PS II complex exhibited high oxygen evolution (879 mu mol O-2. mg(-1) Chl. h(-1)) in the present of 2.5 mM DCIP as an artificial acceptor and was active in photoreduction of DCIP (2, 6- dichloroindophenol) by DPC (1, 5- diphenylcarbazide) at 104 ueq. mg(-1).Chl a. h(-1). Extrinsic proteins of P. haitanesis PS II consisted of 33, 20, 16, 12 kD proteins and cyt c-550 (15 kD), and were different with that of cyanobacteria and higher plants.; Thylakoid membranes were isolated and purified from gametophyte of Porphyra haitanesis by sucrose density gradient ultracentrifugation. After P. haitanesis gametophyte thylakoid membranes were solubilized with sodium dodecyl sulfate (SDS), the highly pure and active photosystem (PS) II complex was isolated and characterized by the room absorption and fluorescence emission spectra, DCIP (2, 6-Dichloroindophenol) reduction and oxygen- evolving rates. The PS II complex from P. haitanesis had the characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 urn) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Acquired PS II complex exhibited high oxygen evolution (879 mu mol O-2. mg(-1) Chl. h(-1)) in the present of 2.5 mM DCIP as an artificial acceptor and was active in photoreduction of DCIP (2, 6- dichloroindophenol) by DPC (1, 5- diphenylcarbazide) at 104 ueq. mg(-1).Chl a. h(-1). Extrinsic proteins of P. haitanesis PS II consisted of 33, 20, 16, 12 kD proteins and cyt c-550 (15 kD), and were different with that of cyanobacteria and higher plants.
关键词	Porphyra Haitanesis Thylakoid Membranes Photosystem Ii
学科领域	Oceanography
收录类别	SCI
语种	英语
WOS研究方向	Oceanography
WOS类目	Oceanography
WOS记录号	WOS:000317453500002
WOS关键词	EXTRINSIC PROTEINS ; THYLAKOID MEMBRANES ; CYANIDIUM-CALDARIUM ; RECONSTITUTION ; POLYPEPTIDES ; YEZOENSIS ; BINDING ; PSII
WOS标题词	Science & Technology ; Physical Sciences
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16599
专题	实验海洋生物学重点实验室
通讯作者	Li, YX
作者单位	1.Nanyang Normal Univ, Sch Life Sci & Technol, Nanyang 473061, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Nation Deep Sea Ctr State Ocean Adm, Qingdao, Peoples R China 4.Shandong Univ Technol, Sch Life Sci, Zibo, Peoples R China 5.Jimei Univ, Dept Aquaculture, Inst Fishery Biotechnol, Xiamen 361021, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Li, Ying Xia,Wang, Guang Ce,Niu, Jian Feng,et al. Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis[J]. INDIAN JOURNAL OF GEO-MARINE SCIENCES,2013,42(1):11-15.
APA	Li, Ying Xia,Wang, Guang Ce,Niu, Jian Feng,Gao, Zheng Quan,Chen, Chang Sheng,&Li, YX.(2013).Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis.INDIAN JOURNAL OF GEO-MARINE SCIENCES,42(1),11-15.
MLA	Li, Ying Xia,et al."Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis".INDIAN JOURNAL OF GEO-MARINE SCIENCES 42.1(2013):11-15.

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