Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Modulation of haemocyte phagocytic and antibacterial activity by alpha-adrenergic receptor in scallop Chlamys farreri | |
Zhou, Zhi1; Jiang, Qiufeng1,2; Wang, Mengqiang1 ; Yue, Feng1,2; Wang, Lingling1; Wang, Leilei1,2; Li, Fengmei1; Liu, Rui1; Song, Linsheng1; Wang, LL
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| 2013-09-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 35期号:3页码:825-832 |
| 文章类型 | Article |
| 摘要 | The adrenergic receptors are a class of G protein-coupled receptors, through which norepinephrine and epinephrine trigger the second messenger to modulate the immune response in immunocytes of vertebrate. In the present study, a gene coding the homologue of a-adrenergic receptor was identified from scallop Chlamys farreri (designated Cf alpha AR). Its deduced protein comprised 318 amino acids, and contained a conserved 7tm_1 domain. After Cf alpha AR protein was expressed in the HEK293 cells, the stimulation of octopamine, tyramine, epinephrine and isoprenaline (beta-adrenergic receptor agonist) did not change significantly the intracellular cAMP concentration, whereas the stimulation of norepinephrine and phenylephrine (a-adrenergic receptor agonist) lowered significantly the cAMP level to 0.52 and 0.84 pmol mu l(-1) (P < 0.05), respectively. The Cf alpha AR transcripts were ubiquitously detected in the tested tissues including haemocytes, adductor muscle, kidney, hepatopancreas, gill, gonad and mantle, with the highest expression in the gill. The expression level of Cf alpha AR mRNA decreased significantly (0.21-fold, P < 0.05) at 3 h after the challenge of bacteria Vibrio anguillarum. Then, it began to increase (4.74-fold, P < 0.05) at 12 h, and reached the highest level (4.92-fold, P < 0.05) at 24 h after bacteria challenge. The addition of a-adrenergic receptor agonist to the primary scallop haemocytes repressed significantly the increase of phagocytic and antibacterial activity induced by LPS stimulation, while the induction was reverted by the addition of a-adrenergic receptor antagonist. These results collectively suggested that alpha-adrenergic receptor could be regulated dynamically in the transcriptional level during the immune response, and it could modulate the haemocyte phagocytic and antibacterial function through the second messenger CAMP, which might be requisite for pathogen elimination and the homeostasis maintenance in scallop. (C) 2013 Elsevier Ltd. All rights reserved.; The adrenergic receptors are a class of G protein-coupled receptors, through which norepinephrine and epinephrine trigger the second messenger to modulate the immune response in immunocytes of vertebrate. In the present study, a gene coding the homologue of a-adrenergic receptor was identified from scallop Chlamys farreri (designated Cf alpha AR). Its deduced protein comprised 318 amino acids, and contained a conserved 7tm_1 domain. After Cf alpha AR protein was expressed in the HEK293 cells, the stimulation of octopamine, tyramine, epinephrine and isoprenaline (beta-adrenergic receptor agonist) did not change significantly the intracellular cAMP concentration, whereas the stimulation of norepinephrine and phenylephrine (a-adrenergic receptor agonist) lowered significantly the cAMP level to 0.52 and 0.84 pmol mu l(-1) (P < 0.05), respectively. The Cf alpha AR transcripts were ubiquitously detected in the tested tissues including haemocytes, adductor muscle, kidney, hepatopancreas, gill, gonad and mantle, with the highest expression in the gill. The expression level of Cf alpha AR mRNA decreased significantly (0.21-fold, P < 0.05) at 3 h after the challenge of bacteria Vibrio anguillarum. Then, it began to increase (4.74-fold, P < 0.05) at 12 h, and reached the highest level (4.92-fold, P < 0.05) at 24 h after bacteria challenge. The addition of a-adrenergic receptor agonist to the primary scallop haemocytes repressed significantly the increase of phagocytic and antibacterial activity induced by LPS stimulation, while the induction was reverted by the addition of a-adrenergic receptor antagonist. These results collectively suggested that alpha-adrenergic receptor could be regulated dynamically in the transcriptional level during the immune response, and it could modulate the haemocyte phagocytic and antibacterial function through the second messenger CAMP, which might be requisite for pathogen elimination and the homeostasis maintenance in scallop. (C) 2013 Elsevier Ltd. All rights reserved. |
| 关键词 | Alpha-adrenergic Receptor Immunomodulation Camp Haemocyte Scallop |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2013.06.020 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000324511700024 |
| WOS关键词 | FUNCTIONAL-CHARACTERIZATION ; MOLECULAR CHARACTERIZATION ; IMMUNE-RESPONSE ; BIVALVE MOLLUSK ; STRESS-RESPONSE ; MYTILUS-EDULIS ; HOST-DEFENSE ; NORADRENALINE ; BETA ; PROTEIN |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16604 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, LL |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhou, Zhi,Jiang, Qiufeng,Wang, Mengqiang,et al. Modulation of haemocyte phagocytic and antibacterial activity by alpha-adrenergic receptor in scallop Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):825-832. |
| APA | Zhou, Zhi.,Jiang, Qiufeng.,Wang, Mengqiang.,Yue, Feng.,Wang, Lingling.,...&Wang, LL.(2013).Modulation of haemocyte phagocytic and antibacterial activity by alpha-adrenergic receptor in scallop Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,35(3),825-832. |
| MLA | Zhou, Zhi,et al."Modulation of haemocyte phagocytic and antibacterial activity by alpha-adrenergic receptor in scallop Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):825-832. |
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| Modulation of haemoc（1508KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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