Screening of genes regulated by relish in Chinese shrimp Fenneropenaeus chinensis

doi:10.1016/j.dci.2013.06.003

IOCAS-IR > 实验海洋生物学重点实验室

	Screening of genes regulated by relish in Chinese shrimp Fenneropenaeus chinensis
	Wang, Dongdong 1,2; Li, Shihao 1; Li, Fuhua 1; Li, FH
	2013-10-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	41 期号:2 页码:209-216
文章类型	Article
摘要	Relish is a key NF-kappa B transcription factor in the innate immunity. Learning the function of Relish in regulating the related genes of shrimp will be helpful to understand the shrimp immunity. In the present study, RNA interference (RNAi) and suppression subtractive hybridization (SSH) techniques were combined together to identify the genes regulated by Relish. A forward SSH library represents the genes whose transcription was regulated by Relish, and the reverse SSH library represents the genes whose transcription was up-regulated after Relish was silenced in shrimp responsive to Vibrio anguillarium (VA) stimulation. In the forward library, 43 unique genes were identified, and in the reverse library, 57 genes were identified. The expression of ten differentially expressed genes, including early cuticle protein5 (ECP5), Toll-like receptor protein (TLRP), antiviral factor (AV), C-type lectin receptor (CLR), thrombospondin (TSP), S-adenosylmethionine synthetase (SAMS), carcinolectin 5b-5 (CL5b-5), QM protein (QMP), heat shock protein 67B2 (HSP67B2), and Thioredoxin-related protein 14 (TRP14) were further confirmed by real-time PCR. The present data provides us a wide view to understand the function of Relish gene in the innate immunity of shrimp. (C) 2013 Elsevier Ltd. All rights reserved.; Relish is a key NF-kappa B transcription factor in the innate immunity. Learning the function of Relish in regulating the related genes of shrimp will be helpful to understand the shrimp immunity. In the present study, RNA interference (RNAi) and suppression subtractive hybridization (SSH) techniques were combined together to identify the genes regulated by Relish. A forward SSH library represents the genes whose transcription was regulated by Relish, and the reverse SSH library represents the genes whose transcription was up-regulated after Relish was silenced in shrimp responsive to Vibrio anguillarium (VA) stimulation. In the forward library, 43 unique genes were identified, and in the reverse library, 57 genes were identified. The expression of ten differentially expressed genes, including early cuticle protein5 (ECP5), Toll-like receptor protein (TLRP), antiviral factor (AV), C-type lectin receptor (CLR), thrombospondin (TSP), S-adenosylmethionine synthetase (SAMS), carcinolectin 5b-5 (CL5b-5), QM protein (QMP), heat shock protein 67B2 (HSP67B2), and Thioredoxin-related protein 14 (TRP14) were further confirmed by real-time PCR. The present data provides us a wide view to understand the function of Relish gene in the innate immunity of shrimp. (C) 2013 Elsevier Ltd. All rights reserved.
关键词	Relish Rnai Ssh Shrimp Immunity
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2013.06.003
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
WOS记录号	WOS:000323870800011
WOS关键词	BLACK TIGER SHRIMP ; SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; S-ADENOSYLMETHIONINE SYNTHETASE ; ANTIMICROBIAL PEPTIDE GENES ; IMMUNE-RELATED GENES ; SPOT SYNDROME VIRUS ; NF-KAPPA-B ; PENAEUS-MONODON ; LITOPENAEUS-VANNAMEI ; EXPRESSION PROFILES
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16625
专题	实验海洋生物学重点实验室
通讯作者	Li, FH
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, Dongdong,Li, Shihao,Li, Fuhua,et al. Screening of genes regulated by relish in Chinese shrimp Fenneropenaeus chinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,41(2):209-216.
APA	Wang, Dongdong,Li, Shihao,Li, Fuhua,&Li, FH.(2013).Screening of genes regulated by relish in Chinese shrimp Fenneropenaeus chinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,41(2),209-216.
MLA	Wang, Dongdong,et al."Screening of genes regulated by relish in Chinese shrimp Fenneropenaeus chinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 41.2(2013):209-216.

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