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| The immunomodulation of inducible nitric oxide in scallop Chlamys farreri | |
Jiang, Qiufen1,2; Zhou, Zhi1,2; Wang, Lingling1; Shi, Xiaowei1,2; Wang, Jingjing1,2 ; Yue, Feng1,2; Yi, Qilin1,2; Yang, Chuanyan1,2; Song, Linsheng1; Wang, LL
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| 2013 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 34期号:1页码:100-108 |
| 文章类型 | Article |
| 摘要 | Nitric oxide (NO) is an important signalling molecule which plays an indispensable role in immunity of all vertebrates and invertebrates. In the present study, the immunomodulation of inducible NO in scallop Chlamys farreri was examined by monitoring the alterations of haemocyte behaviours and related immune molecules in response to the stimulations of LPS and/or with S-Methylisothiourea Sulphate (SMT), an inhibitor of inducible NO synthase (NOS). The total activity of NOS and NO concentration in the haemolymph of scallop C. farreri increased significantly at 3,6 and 12 h after LPS stimulation respectively, whereas their increases were fully repressed when scallops were treated in the collaborating of LPS and SMT. Meanwhile, some cellular and humoral immune parameters were determined after the stimulation of LPS and SMT to investigate the role of inducible NO in innate immunity of scallop. After LPS stimulation, the highest levels of haemocytes apoptosis and phagocytosis were observed at 24 h (38.5 +/- 2.5%, P < 0.01) and 12 h (38.6 +/- 0.2%, P < 0.01), respectively, and the reactive oxygen species (ROS) level (5.88 +/- 0.90%, P < 0.01) of haemocytes and anti-bacterial activity of haemolymph (10.0 +/- 2.2%, P < 0.01) all elevated dramatically at 12 h. Although the activity of lysozyme and phenoloxidase (PO) in haemolymph both declined at 48 h (93.0 +/- 6.3 U mgprot(-1), 0.40 +/- 0.06 U mgprot(-1), P < 0.01), superoxide dismutase (SOD) activity and GSH concentration both increased to the highest level at 24 h post treatment (99.2 +/- 8.1 U mgprot(-1), 93.0 +/- 63 nmol mgprot(-1), P < 0.01). After the collaborating treatment of LPS and SMT, the apoptosis index increased much higher from 48 h, while the increase of haemocytes phagocytosis, ROS level and haemolymph anti-bacteria activities were suppressed completely at 12 h. The declines of lysozyme and PO activity in haemolymph were reversed at 48 h, and the rise of SOD activity and GSH concentration started earlier from 3 h. These results indicated clearly that NO could participate in the scallop immunity and play a crucial role in the modulation of immune response including haemocytes apoptosis and phagocytosis, anti-bacterial activity and redox homeostasis in the haemolymph of scallop. (c) 2012 Elsevier Ltd. All rights reserved.; Nitric oxide (NO) is an important signalling molecule which plays an indispensable role in immunity of all vertebrates and invertebrates. In the present study, the immunomodulation of inducible NO in scallop Chlamys farreri was examined by monitoring the alterations of haemocyte behaviours and related immune molecules in response to the stimulations of LPS and/or with S-Methylisothiourea Sulphate (SMT), an inhibitor of inducible NO synthase (NOS). The total activity of NOS and NO concentration in the haemolymph of scallop C. farreri increased significantly at 3,6 and 12 h after LPS stimulation respectively, whereas their increases were fully repressed when scallops were treated in the collaborating of LPS and SMT. Meanwhile, some cellular and humoral immune parameters were determined after the stimulation of LPS and SMT to investigate the role of inducible NO in innate immunity of scallop. After LPS stimulation, the highest levels of haemocytes apoptosis and phagocytosis were observed at 24 h (38.5 +/- 2.5%, P < 0.01) and 12 h (38.6 +/- 0.2%, P < 0.01), respectively, and the reactive oxygen species (ROS) level (5.88 +/- 0.90%, P < 0.01) of haemocytes and anti-bacterial activity of haemolymph (10.0 +/- 2.2%, P < 0.01) all elevated dramatically at 12 h. Although the activity of lysozyme and phenoloxidase (PO) in haemolymph both declined at 48 h (93.0 +/- 6.3 U mgprot(-1), 0.40 +/- 0.06 U mgprot(-1), P < 0.01), superoxide dismutase (SOD) activity and GSH concentration both increased to the highest level at 24 h post treatment (99.2 +/- 8.1 U mgprot(-1), 93.0 +/- 63 nmol mgprot(-1), P < 0.01). After the collaborating treatment of LPS and SMT, the apoptosis index increased much higher from 48 h, while the increase of haemocytes phagocytosis, ROS level and haemolymph anti-bacteria activities were suppressed completely at 12 h. The declines of lysozyme and PO activity in haemolymph were reversed at 48 h, and the rise of SOD activity and GSH concentration started earlier from 3 h. These results indicated clearly that NO could participate in the scallop immunity and play a crucial role in the modulation of immune response including haemocytes apoptosis and phagocytosis, anti-bacterial activity and redox homeostasis in the haemolymph of scallop. (c) 2012 Elsevier Ltd. All rights reserved. |
| 关键词 | Chlamys Farreri Nitric Oxide (No) No Synthase (Nos) Immunity Lps S-methylisothiourea Sulphate (Smt) |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2012.10.011 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000314743600012 |
| WOS关键词 | MYTILUS-GALLOPROVINCIALIS HEMOCYTES ; INVERTEBRATE IMMUNOCYTES ; LYMNAEA-STAGNALIS ; NO PRODUCTION ; POND SNAIL ; L-ARGININE ; IN-VITRO ; CELLS ; SYNTHASE ; SUPEROXIDE |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16648 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, LL |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Jiang, Qiufen,Zhou, Zhi,Wang, Lingling,et al. The immunomodulation of inducible nitric oxide in scallop Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(1):100-108. |
| APA | Jiang, Qiufen.,Zhou, Zhi.,Wang, Lingling.,Shi, Xiaowei.,Wang, Jingjing.,...&Wang, LL.(2013).The immunomodulation of inducible nitric oxide in scallop Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,34(1),100-108. |
| MLA | Jiang, Qiufen,et al."The immunomodulation of inducible nitric oxide in scallop Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 34.1(2013):100-108. |
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