Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Three EST-SSR Markers Associated with QTL for the Growth of the Clam Meretrix meretrix Revealed by Selective Genotyping | |
Lu, Xia1,2; Wang, Hongxia1; Liu, Baozhong1 ; Xiang, Jianhai1; Liu, BZ
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| 2013-02-01 | |
| 发表期刊 | MARINE BIOTECHNOLOGY
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| ISSN | 1436-2228 |
| 卷号 | 15期号:1页码:16-25 |
| 文章类型 | Article |
| 摘要 | The clam Meretrix meretrix is a member of widely cultured, commercially important clams. A marker-trait association analysis was performed using expressed sequence tag (EST) simple sequence repeat (SSR) markers for marker-assisted selection in M. meretrix. Three markers, MM1272, MM2034, and MM7721, were found to be significantly associated with quantitative trait loci (QTLs) controlling shell length (P < 0.0001) in clams of a fast-growing population (JSF) and a control population (JSC). The 144-bp allele of MM1272, the 154-bp allele of MM2034, and the 152- and 165-bp alleles of MM7721 showed a significantly higher frequency in the JSF population (17.65, 36.41, 28.67, and 29.33 %) than in the JSC population (4.65, 8.33, 3.47, and 5.56 %). The three markers showed lower values for the number of alleles and observed heterozygosity as well as a higher proportion of homozygotes in JSF than in JSC population. The three markers have been further confirmed in the high and low tails of another population (09G(3)SPSB); similarly, lower values for the number of alleles and observed heterozygosity as well as a higher proportion of homozygotes were found in 09G(3)SPSB(H). The putative functions of the three gene fragments containing MM1272, MM2034, and MM7721 also suggested that the three SSR-containing genes might be involved in growth of M. meretrix. All the results suggest that the three EST-SSR markers associated with growth QTLs would be useful for marker-assisted selection in M. meretrix breeding.; The clam Meretrix meretrix is a member of widely cultured, commercially important clams. A marker-trait association analysis was performed using expressed sequence tag (EST) simple sequence repeat (SSR) markers for marker-assisted selection in M. meretrix. Three markers, MM1272, MM2034, and MM7721, were found to be significantly associated with quantitative trait loci (QTLs) controlling shell length (P < 0.0001) in clams of a fast-growing population (JSF) and a control population (JSC). The 144-bp allele of MM1272, the 154-bp allele of MM2034, and the 152- and 165-bp alleles of MM7721 showed a significantly higher frequency in the JSF population (17.65, 36.41, 28.67, and 29.33 %) than in the JSC population (4.65, 8.33, 3.47, and 5.56 %). The three markers showed lower values for the number of alleles and observed heterozygosity as well as a higher proportion of homozygotes in JSF than in JSC population. The three markers have been further confirmed in the high and low tails of another population (09G(3)SPSB); similarly, lower values for the number of alleles and observed heterozygosity as well as a higher proportion of homozygotes were found in 09G(3)SPSB(H). The putative functions of the three gene fragments containing MM1272, MM2034, and MM7721 also suggested that the three SSR-containing genes might be involved in growth of M. meretrix. All the results suggest that the three EST-SSR markers associated with growth QTLs would be useful for marker-assisted selection in M. meretrix breeding. |
| 关键词 | Meretrix Meretrix Est-ssr Qtl Selective Genotyping Marker-trait Association |
| 学科领域 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| DOI | 10.1007/s10126-012-9453-4 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS类目 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000312740300002 |
| WOS关键词 | QUANTITATIVE-TRAIT LOCI ; TROUT ONCORHYNCHUS-MYKISS ; LINKAGE DISEQUILIBRIUM ; MICROSATELLITE MARKERS ; SEGREGANT ANALYSIS ; GENETIC DIVERSITY ; MOLECULAR-MARKER ; GENOMIC REGIONS ; GRAIN-YIELD ; MAIZE |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16662 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, BZ |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Lu, Xia,Wang, Hongxia,Liu, Baozhong,et al. Three EST-SSR Markers Associated with QTL for the Growth of the Clam Meretrix meretrix Revealed by Selective Genotyping[J]. MARINE BIOTECHNOLOGY,2013,15(1):16-25. |
| APA | Lu, Xia,Wang, Hongxia,Liu, Baozhong,Xiang, Jianhai,&Liu, BZ.(2013).Three EST-SSR Markers Associated with QTL for the Growth of the Clam Meretrix meretrix Revealed by Selective Genotyping.MARINE BIOTECHNOLOGY,15(1),16-25. |
| MLA | Lu, Xia,et al."Three EST-SSR Markers Associated with QTL for the Growth of the Clam Meretrix meretrix Revealed by Selective Genotyping".MARINE BIOTECHNOLOGY 15.1(2013):16-25. |
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