Three vibrio-resistance related EST-SSR markers revealed by selective genotyping in the clam Meretrix meretrix

doi:10.1016/j.fsi.2013.04.046

IOCAS-IR > 实验海洋生物学重点实验室

	Three vibrio-resistance related EST-SSR markers revealed by selective genotyping in the clam Meretrix meretrix
	Nie, Qing1,2 ; Yue, Xin 1; Chai, Xueliang 3; Wang, Hongxia 1; Liu, Baozhong1 ; Liu, BZ
	2013-08-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	35 期号:2 页码:421-428
文章类型	Article
摘要	The clam Meretrix meretrix is an important commercial bivalve distributed in the coastal areas of South and Southeast Asia. In this study, marker trait association analyses were performed based on the stock materials of M. meretrix with different vibrio-resistance profile obtained by selective breeding. Forty-eight EST-SSR markers were screened and 27 polymorphic SSRs of them were genotyped in the clam stocks with different resistance to Vibrio parahaemolyticus (11-R and 11-S) and to Vibrio harveyi (09-R and 09-C). Allele frequency distributions of the SSRs among different stocks were compared using Pearson's Chi-square test, and three functional EST-SSR markers (MM959, MM4765 and MM8364) were found to be associated with vibrio-resistance trait. The 140-bp allele of MM959 and 128-bp allele of MM4765 had significantly higher frequencies in resistant groups (11-R and 09-R) than in susceptive/control groups (11-S and 09-C) (P < 0.01), which suggested that the clams carrying these two alleles have stronger resistance against vibrio. Clam individuals of 11-S were divided into three subgroups based on the survival time post-challenge, and the multi-dimensional scaling (MDS) analysis showed that clusters generated by genetic similarity revealed by the three SSR markers were consistent with the three subgroups distinctions. The putative functions of contig959, contig4765 and contig8364 also suggested that the three SSR-involved genes might play important roles in immunity of M. meretrix. All these results supported that EST-SSR markers MM959, MM4765 and MM8364 were associated with vibrio-resistance and would be useful for marker-assisted selection (MAS) in M. meretrix genetic breeding. (C) 2013 Elsevier Ltd. All rights reserved.; The clam Meretrix meretrix is an important commercial bivalve distributed in the coastal areas of South and Southeast Asia. In this study, marker trait association analyses were performed based on the stock materials of M. meretrix with different vibrio-resistance profile obtained by selective breeding. Forty-eight EST-SSR markers were screened and 27 polymorphic SSRs of them were genotyped in the clam stocks with different resistance to Vibrio parahaemolyticus (11-R and 11-S) and to Vibrio harveyi (09-R and 09-C). Allele frequency distributions of the SSRs among different stocks were compared using Pearson's Chi-square test, and three functional EST-SSR markers (MM959, MM4765 and MM8364) were found to be associated with vibrio-resistance trait. The 140-bp allele of MM959 and 128-bp allele of MM4765 had significantly higher frequencies in resistant groups (11-R and 09-R) than in susceptive/control groups (11-S and 09-C) (P < 0.01), which suggested that the clams carrying these two alleles have stronger resistance against vibrio. Clam individuals of 11-S were divided into three subgroups based on the survival time post-challenge, and the multi-dimensional scaling (MDS) analysis showed that clusters generated by genetic similarity revealed by the three SSR markers were consistent with the three subgroups distinctions. The putative functions of contig959, contig4765 and contig8364 also suggested that the three SSR-involved genes might play important roles in immunity of M. meretrix. All these results supported that EST-SSR markers MM959, MM4765 and MM8364 were associated with vibrio-resistance and would be useful for marker-assisted selection (MAS) in M. meretrix genetic breeding. (C) 2013 Elsevier Ltd. All rights reserved.
关键词	Meretrix Meretrix Est-ssr Marker-trait Association Vibrio-resistance
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2013.04.046
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000322207500027
WOS关键词	QUANTITATIVE-TRAIT LOCI ; FLOUNDER PARALICHTHYS-OLIVACEUS ; GENETIC-MARKERS ; ASSISTED SELECTION ; MOLECULAR CHARACTERIZATION ; DISEASE ; LINKAGE ; GROWTH ; QTL ; ASSOCIATION
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16663
专题	实验海洋生物学重点实验室
通讯作者	Liu, BZ
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Zhejiang Mariculture Res Inst, Wenzhou 325005, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Nie, Qing,Yue, Xin,Chai, Xueliang,et al. Three vibrio-resistance related EST-SSR markers revealed by selective genotyping in the clam Meretrix meretrix[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(2):421-428.
APA	Nie, Qing,Yue, Xin,Chai, Xueliang,Wang, Hongxia,Liu, Baozhong,&Liu, BZ.(2013).Three vibrio-resistance related EST-SSR markers revealed by selective genotyping in the clam Meretrix meretrix.FISH & SHELLFISH IMMUNOLOGY,35(2),421-428.
MLA	Nie, Qing,et al."Three vibrio-resistance related EST-SSR markers revealed by selective genotyping in the clam Meretrix meretrix".FISH & SHELLFISH IMMUNOLOGY 35.2(2013):421-428.

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