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| Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Hepatopancreas of Microbial Challenged Mitten Crab Eriocheir sinensis | |
Li, Xihong1,2; Cui, Zhaoxia1,3; Liu, Yuan1; Song, Chengwen1,2 ; Shi, Guohui1,2; Cui, ZX
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| 2013-07-17 | |
| 发表期刊 | PLOS ONE
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| ISSN | 1932-6203 |
| 卷号 | 8期号:7页码:e68233 |
| 文章类型 | Article |
| 摘要 | Background: The Chinese mitten crab Eriocheir sinensis is an important economic crustacean and has been seriously attacked by various diseases, which requires more and more information for immune relevant genes on genome background. Recently, high-throughput RNA sequencing (RNA-seq) technology provides a powerful and efficient method for transcript analysis and immune gene discovery.; Background: The Chinese mitten crab Eriocheir sinensis is an important economic crustacean and has been seriously attacked by various diseases, which requires more and more information for immune relevant genes on genome background. Recently, high-throughput RNA sequencing (RNA-seq) technology provides a powerful and efficient method for transcript analysis and immune gene discovery. Methods/Principal Findings: A cDNA library from hepatopancreas of E. sinensis challenged by a mixture of three pathogen strains (Gram-positive bacteria Micrococcus luteus, Gram-negative bacteria Vibrio alginolyticus and fungi Pichia pastoris; 10 8 cfu.mL(-1)) was constructed and randomly sequenced using Illumina technique. Totally 39.76 million clean reads were assembled to 70,300 unigenes. After ruling out short-length and low-quality sequences, 52,074 non-redundant unigenes were compared to public databases for homology searching and 17,617 of them showed high similarity to sequences in NCBI non-redundant protein (Nr) database. For function classification and pathway assignment, 18,734 (36.00%) unigenes were categorized to three Gene Ontology (GO) categories, 12,243 (23.51%) were classified to 25 Clusters of Orthologous Groups (COG), and 8,983 (17.25%) were assigned to six Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Potentially, 24, 14, 47 and 132 unigenes were characterized to be involved in Toll, IMD, JAK-STAT and MAPK pathways, respectively. Conclusions/Significance: This is the first systematical transcriptome analysis of components relating to innate immune pathways in E. sinensis. Functional genes and putative pathways identified here will contribute to better understand immune system and prevent various diseases in crab. |
| 学科领域 | Science & Technology - Other Topics |
| DOI | 10.1371/journal.pone.0068233 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000322000600006 |
| WOS关键词 | ANTIMICROBIAL PEPTIDE GENES ; LITOPENAEUS-VANNAMEI ; RNA-SEQ ; ANTILIPOPOLYSACCHARIDE FACTOR ; SIGNALING PATHWAYS ; WSSV INFECTION ; TREMOR DISEASE ; WHITE SHRIMP ; IDENTIFICATION ; EXPRESSION |
| WOS标题词 | Science & Technology |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16664 |
| 专题 | 海洋生态与环境科学重点实验室 实验海洋生物学重点实验室 |
| 通讯作者 | Cui, ZX |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Xihong,Cui, Zhaoxia,Liu, Yuan,et al. Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Hepatopancreas of Microbial Challenged Mitten Crab Eriocheir sinensis[J]. PLOS ONE,2013,8(7):e68233. |
| APA | Li, Xihong,Cui, Zhaoxia,Liu, Yuan,Song, Chengwen,Shi, Guohui,&Cui, ZX.(2013).Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Hepatopancreas of Microbial Challenged Mitten Crab Eriocheir sinensis.PLOS ONE,8(7),e68233. |
| MLA | Li, Xihong,et al."Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Hepatopancreas of Microbial Challenged Mitten Crab Eriocheir sinensis".PLOS ONE 8.7(2013):e68233. |
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| Transcriptome Analys（1614KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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