Transcriptome Analysis on Chinese Shrimp Fenneropenaeus chinensis during WSSV Acute Infection

doi:10.1371/journal.pone.0058627

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	Transcriptome Analysis on Chinese Shrimp Fenneropenaeus chinensis during WSSV Acute Infection
	Li, Shihao; Zhang, Xiaojun; Sun, Zheng; Li, Fuhua; Xiang, Jianhai; Li, FH
	2013-03-19
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:3 页码:e58627
文章类型	Article
摘要	Previous studies have discovered a lot of immune-related genes responding to white spot syndrome virus (WSSV) infection in crustacean. However, little information is available in relation to underlying mechanisms of host responses during the WSSV acute infection stage in naturally infected shrimp. In this study, we employed next-generation sequencing and bioinformatic techniques to observe the transcriptome differences of the shrimp between latent infection stage and acute infection stage. A total of 64,188,426 Illumina reads, including 31,685,758 reads from the latent infection group and 32,502,668 reads from the acute infection group, were generated and assembled into 46,676 unigenes (mean length: 676 bp; range: 200-15,094 bp). Approximately 24,000 peptides were predicted and classified based on homology searches, gene ontology, clusters of orthologous groups of proteins, and biological pathway mapping. Among which, 805 differentially expressed genes were identified and categorized into 11 groups based on their possible function. Genes in the Toll and IMD pathways, the Ras-activated endocytosis process, the RNA interference pathway, anti-lipopolysaccharide factors and many other genes, were found to be activated in shrimp from latent infection stage to acute infection stage. The anti-bacterially proPO-activating cascade was firstly uncovered to be probably participated in antiviral process. These genes contain not only members playing function in host defense against WSSV, but also genes utilized by WSSV for its rapid proliferation. In addition, the transcriptome data provides detail information for identifying novel genes in absence of the genome database of shrimp.; Previous studies have discovered a lot of immune-related genes responding to white spot syndrome virus (WSSV) infection in crustacean. However, little information is available in relation to underlying mechanisms of host responses during the WSSV acute infection stage in naturally infected shrimp. In this study, we employed next-generation sequencing and bioinformatic techniques to observe the transcriptome differences of the shrimp between latent infection stage and acute infection stage. A total of 64,188,426 Illumina reads, including 31,685,758 reads from the latent infection group and 32,502,668 reads from the acute infection group, were generated and assembled into 46,676 unigenes (mean length: 676 bp; range: 200-15,094 bp). Approximately 24,000 peptides were predicted and classified based on homology searches, gene ontology, clusters of orthologous groups of proteins, and biological pathway mapping. Among which, 805 differentially expressed genes were identified and categorized into 11 groups based on their possible function. Genes in the Toll and IMD pathways, the Ras-activated endocytosis process, the RNA interference pathway, anti-lipopolysaccharide factors and many other genes, were found to be activated in shrimp from latent infection stage to acute infection stage. The anti-bacterially proPO-activating cascade was firstly uncovered to be probably participated in antiviral process. These genes contain not only members playing function in host defense against WSSV, but also genes utilized by WSSV for its rapid proliferation. In addition, the transcriptome data provides detail information for identifying novel genes in absence of the genome database of shrimp.
学科领域	Science & Technology - Other Topics
DOI	10.1371/journal.pone.0058627
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000317562100033
WOS关键词	SPOT-SYNDROME-VIRUS ; BLACK TIGER SHRIMP ; DIFFERENTIALLY EXPRESSED GENES ; SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; PROPHENOLOXIDASE-ACTIVATING SYSTEM ; ANTILIPOPOLYSACCHARIDE FACTOR ALF ; ANTIMICROBIAL PEPTIDE GENES ; POLYMERASE-CHAIN-REACTION ; RNA INTERFERENCE PATHWAY ; CRAB ERIOCHEIR-SINENSIS
WOS标题词	Science & Technology
引用统计	被引频次：156[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16666
专题	实验海洋生物学重点实验室
通讯作者	Li, FH
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Shihao,Zhang, Xiaojun,Sun, Zheng,et al. Transcriptome Analysis on Chinese Shrimp Fenneropenaeus chinensis during WSSV Acute Infection[J]. PLOS ONE,2013,8(3):e58627.
APA	Li, Shihao,Zhang, Xiaojun,Sun, Zheng,Li, Fuhua,Xiang, Jianhai,&Li, FH.(2013).Transcriptome Analysis on Chinese Shrimp Fenneropenaeus chinensis during WSSV Acute Infection.PLOS ONE,8(3),e58627.
MLA	Li, Shihao,et al."Transcriptome Analysis on Chinese Shrimp Fenneropenaeus chinensis during WSSV Acute Infection".PLOS ONE 8.3(2013):e58627.

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