| Cloning of the cDNA encoding adenosine 5'-monophosphate deaminase 1 and its mRNA expression in Japanese flounder Paralichthys olivaceus | |
Jiang Keyong1,2; Sun Shujuan1; Liu Mei1; Wang Baojie1; Meng Xiaolin1,2; Wang Lei1; Wang, L
| |
| 2013 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| ISSN | 0254-4059 |
| 卷号 | 31期号:1页码:118-127 |
| 文章类型 | Article |
| 摘要 | AMP deaminase catalyzes the conversion of AMP into IMP and ammonia. In the present study, a full-length cDNA of AMPD1 from skeletal muscle of Japanese flounder Paralichthys olivaceus was cloned and characterized. The 2 526 bp cDNA contains a 5'-UTR of 78 bp, a 3'-UTR of 237 bp and an open reading frame (ORF) of 2 211 bp, which encodes a protein of 736 amino acids. The predicted protein contains a highly conserved AMP deaminase motif (SLSTDDP) and an ATP-binding site sequence (EPLMEEYAIAAQVFK). Phylogenetic analysis showed that the AMPD1 and AMPD3 genes originate from the same branch, but are evolutionarily distant from the AMPD2 gene. RT-PCR showed that the flounder AMPD1 gene was expressed only in skeletal muscle. QRT-PCR analysis revealed a statistically significant 2.54 fold higher level of AMPD1 mRNA in adult muscle (750 +/- 40 g) compared with juvenile muscle (7.5 +/- 2 g) (P < 0.05). HPLC analysis showed that the IMP content in adult muscle (3.35 +/- 0.21 mg/g) was also statistically significantly higher than in juvenile muscle (1.08 +/- 0.04 mg/g) (P < 0.05). There is a direct relationship between the AMPD1 gene expression level and IMP content in the skeletal muscle of juvenile and adult flounders. These results may provide useful information for quality improvement and molecular breeding of aquatic animals.; AMP deaminase catalyzes the conversion of AMP into IMP and ammonia. In the present study, a full-length cDNA of AMPD1 from skeletal muscle of Japanese flounder Paralichthys olivaceus was cloned and characterized. The 2 526 bp cDNA contains a 5'-UTR of 78 bp, a 3'-UTR of 237 bp and an open reading frame (ORF) of 2 211 bp, which encodes a protein of 736 amino acids. The predicted protein contains a highly conserved AMP deaminase motif (SLSTDDP) and an ATP-binding site sequence (EPLMEEYAIAAQVFK). Phylogenetic analysis showed that the AMPD1 and AMPD3 genes originate from the same branch, but are evolutionarily distant from the AMPD2 gene. RT-PCR showed that the flounder AMPD1 gene was expressed only in skeletal muscle. QRT-PCR analysis revealed a statistically significant 2.54 fold higher level of AMPD1 mRNA in adult muscle (750 +/- 40 g) compared with juvenile muscle (7.5 +/- 2 g) (P < 0.05). HPLC analysis showed that the IMP content in adult muscle (3.35 +/- 0.21 mg/g) was also statistically significantly higher than in juvenile muscle (1.08 +/- 0.04 mg/g) (P < 0.05). There is a direct relationship between the AMPD1 gene expression level and IMP content in the skeletal muscle of juvenile and adult flounders. These results may provide useful information for quality improvement and molecular breeding of aquatic animals. |
| 关键词 | Ampd1 Cdna Cloning Mrna Expression Imp Skeletal Muscle Japanese Flounder |
| 学科领域 | Marine & Freshwater Biology ; Oceanography |
| DOI | 10.1007/s00343-013-2055-9 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| WOS类目 | Limnology ; Oceanography |
| WOS记录号 | WOS:000313654000013 |
| WOS关键词 | MUSCLE AMP-DEAMINASE ; SKELETAL-MUSCLE ; MYOADENYLATE DEAMINASE ; RAT TISSUES ; CHROMOSOME-4 ; DEFICIENCY ; ISOZYMES ; FAMILY ; HEART ; FISH |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16690 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Wang, L |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Jiang Keyong,Sun Shujuan,Liu Mei,et al. Cloning of the cDNA encoding adenosine 5'-monophosphate deaminase 1 and its mRNA expression in Japanese flounder Paralichthys olivaceus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(1):118-127. |
| APA | Jiang Keyong.,Sun Shujuan.,Liu Mei.,Wang Baojie.,Meng Xiaolin.,...&Wang, L.(2013).Cloning of the cDNA encoding adenosine 5'-monophosphate deaminase 1 and its mRNA expression in Japanese flounder Paralichthys olivaceus.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(1),118-127. |
| MLA | Jiang Keyong,et al."Cloning of the cDNA encoding adenosine 5'-monophosphate deaminase 1 and its mRNA expression in Japanese flounder Paralichthys olivaceus".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.1(2013):118-127. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Cloning of the cDNA （2099KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论