| Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas | |
Wang, Xiaotong1; Song, Xiaorui1 ; Wang, Tong1; Zhu, Qihui1; Miao, Guoying1; Chen, Yuanxin2; Fang, Xiaodong2; Que, Huayong1; Li, Li1; Zhang, Guofan1; Li, L
| |
| 2013 | |
| 发表期刊 | CURRENT ZOOLOGY
 |
| ISSN | 1674-5507 |
| 卷号 | 59期号:1页码:109-115 |
| 文章类型 | Article |
| 摘要 | Mollusc shell matrix proteins (SMPs) are important functional components embedded in the shell and play a role in shell formation. A SMP (Pif177) was identified previously from the nacreous layer of the Japanese pearl oyster Pinctada fucata, and its cleavage products (named pfPif97 and pfPif80 proteins) were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure between the two species. This homologue (cgPif97) shares characteristics with pfPif97, and suggests that the biological functions of these two proteins may be similar. The expression pattern of cgPif97 in different tissues and development stages indicates that it may play an important role in shell formation of the adult oyster. The morphology of the inner shell surface was affected by injected siRNA of cgPif97 and the calcite laths of the shell became thinner and narrower when the siRNA dose increased, suggesting that the cgPif97 gene plays an important role in calcite shell formation in C. gigas. In conclusion, we found evidence that the Pif177 gene evolved very fast but still retains a similar function among species [Current Zoology 59 (1): 109-115, 2013].; Mollusc shell matrix proteins (SMPs) are important functional components embedded in the shell and play a role in shell formation. A SMP (Pif177) was identified previously from the nacreous layer of the Japanese pearl oyster Pinctada fucata, and its cleavage products (named pfPif97 and pfPif80 proteins) were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure between the two species. This homologue (cgPif97) shares characteristics with pfPif97, and suggests that the biological functions of these two proteins may be similar. The expression pattern of cgPif97 in different tissues and development stages indicates that it may play an important role in shell formation of the adult oyster. The morphology of the inner shell surface was affected by injected siRNA of cgPif97 and the calcite laths of the shell became thinner and narrower when the siRNA dose increased, suggesting that the cgPif97 gene plays an important role in calcite shell formation in C. gigas. In conclusion, we found evidence that the Pif177 gene evolved very fast but still retains a similar function among species [Current Zoology 59 (1): 109-115, 2013]. |
| 关键词 | Mollusca Crassostrea Gigas Pif97 Gene Biomineralization Shell Formation Calcite |
| 学科领域 | Zoology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Zoology |
| WOS类目 | Zoology |
| WOS记录号 | WOS:000312556500009 |
| WOS关键词 | MATRIX PROTEIN ; SHELL ; NACRE ; MOLLUSKS ; CRYSTALS |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16697 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 |
| 通讯作者 | Li, L |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.BGI Shenzhen, Shenzhen 518083, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Xiaotong,Song, Xiaorui,Wang, Tong,et al. Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas[J]. CURRENT ZOOLOGY,2013,59(1):109-115. |
| APA | Wang, Xiaotong.,Song, Xiaorui.,Wang, Tong.,Zhu, Qihui.,Miao, Guoying.,...&Li, L.(2013).Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas.CURRENT ZOOLOGY,59(1),109-115. |
| MLA | Wang, Xiaotong,et al."Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas".CURRENT ZOOLOGY 59.1(2013):109-115. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Evolution and functi（5290KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论