| Extraction and Separation of Fucoidan from Laminaria japonica with Chitosan as Extractant | |
Xing, Ronge ; Liu, Song; Yu, Huahua; Chen, Xiaolin; Qin, Yukun; Li, Kecheng; Li, Pengcheng; Li, PC
| |
| 2013 | |
| 发表期刊 | BIOMED RESEARCH INTERNATIONAL
 |
| ISSN | 2314-6133 |
| 页码 | 193689 |
| 文章类型 | Article |
| 摘要 | Herein the extraction method of fucoidan from Laminaria japonica is reported. Firstly, chitosan, chitosan-N-2-hydroxypropyl trimethyl ammonium chloride (HACC), and hexadecyltrimethylammonium bromide (CPAB) were used to extract the fucoidan. The results showed that chitosan was the optimal extractant compared with the other two extractants. After extraction, different aqueous solutions, including NaCl, KCl, and HCl (pH2), were used to separate fucoidan from chitosan-fucoidan complex. The results showed that the separation ability of NaCl was slightly higher than that of KCl. Moreover, the price of NaCl is lower than that of KCl. Given the quality-price rate, NaCl solution was chosen as the separation solution. Thirdly, the concentration and ratio of NaCl solution : sediment influence the separation of fucoidan from chitosan-fucoidan complex. The results showed that the optimal separation conditions include 4mol/L NaCl solution with the ratio of NaCl solution to sediment at 30:1. Fucoidan content was found to be affected by different separation time. Fucoidan content increased with the increase of separation time, and the optimal separation time was 6 h. Compared with traditional alkali extraction method, this method not only reduces the usage of alkali and acid and alleviate environment pollution, but also has the comparable extraction yield of fucoidan. It is a potential method for extraction of fucoidan.; Herein the extraction method of fucoidan from Laminaria japonica is reported. Firstly, chitosan, chitosan-N-2-hydroxypropyl trimethyl ammonium chloride (HACC), and hexadecyltrimethylammonium bromide (CPAB) were used to extract the fucoidan. The results showed that chitosan was the optimal extractant compared with the other two extractants. After extraction, different aqueous solutions, including NaCl, KCl, and HCl (pH2), were used to separate fucoidan from chitosan-fucoidan complex. The results showed that the separation ability of NaCl was slightly higher than that of KCl. Moreover, the price of NaCl is lower than that of KCl. Given the quality-price rate, NaCl solution was chosen as the separation solution. Thirdly, the concentration and ratio of NaCl solution : sediment influence the separation of fucoidan from chitosan-fucoidan complex. The results showed that the optimal separation conditions include 4mol/L NaCl solution with the ratio of NaCl solution to sediment at 30:1. Fucoidan content was found to be affected by different separation time. Fucoidan content increased with the increase of separation time, and the optimal separation time was 6 h. Compared with traditional alkali extraction method, this method not only reduces the usage of alkali and acid and alleviate environment pollution, but also has the comparable extraction yield of fucoidan. It is a potential method for extraction of fucoidan. |
| 学科领域 | Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
| DOI | 10.1155/2013/193689 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
| WOS类目 | Biotechnology & Applied Microbiology ; Medicine, Research & Experimental |
| WOS记录号 | WOS:000327952600001 |
| WOS关键词 | BROWN SEAWEED ; POLYSACCHARIDES ; NMR |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16698 |
| 专题 | 海洋生物技术研发中心 海洋环境工程技术研究发展中心 |
| 通讯作者 | Li, PC |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Xing, Ronge,Liu, Song,Yu, Huahua,et al. Extraction and Separation of Fucoidan from Laminaria japonica with Chitosan as Extractant[J]. BIOMED RESEARCH INTERNATIONAL,2013:193689. |
| APA | Xing, Ronge.,Liu, Song.,Yu, Huahua.,Chen, Xiaolin.,Qin, Yukun.,...&Li, PC.(2013).Extraction and Separation of Fucoidan from Laminaria japonica with Chitosan as Extractant.BIOMED RESEARCH INTERNATIONAL,193689. |
| MLA | Xing, Ronge,et al."Extraction and Separation of Fucoidan from Laminaria japonica with Chitosan as Extractant".BIOMED RESEARCH INTERNATIONAL (2013):193689. |
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| Extraction and Separ（1130KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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