Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq

doi:10.1007/s10126-019-09942-6

IOCAS-IR > 实验海洋生物学重点实验室

	Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq
	Liu, Youli 1,2,3; Zhu, Qihui1,4 ; Li, Li 1,2,5,6; Wang, Wei 1,5,6,7; Zhang, Guofan 1,5,6,7
	2020-04-01
发表期刊	MARINE BIOTECHNOLOGY
ISSN	1436-2228
卷号	22 期号:2 页码:167-179
通讯作者	Li, Li(lili@qdio.ac.cn)
摘要	The Pacific oyster Crassostrea gigas, a commercially important species inhabiting the intertidal zone, facing enormous temperature fluctuations. Therefore, it is important to identify candidate genes and key regulatory relationships associated with thermal tolerance, which can aid the molecular breeding of oysters. Heat shock transcription factor 1 (HSF1) plays an important role in the thermal stress resistance. However, the regulatory relationship between the expansion of heat shock protein (HSP) HSP 70 and HSF1 is not yet clear in C. gigas. In this study, we analyzed genes regulated by HSF1 in response to heat shock by chromatin immunoprecipitation followed by sequencing (ChIP-seq), determined the expression patterns of target genes by qRT-PCR, and validated the regulatory relationship between one HSP70 and HSF1. We found 916 peaks corresponding to HSF1 binding sites, and these peaks were annotated to the nearest genes. In Gene Ontology analysis, HSF1 target genes were related to signal transduction, energy production, and response to biotic stimulus. Four HSP70 genes, two HSP40 genes, and one small HSP gene exhibited binding to HSF1. One HSP70 with a binding site in the promoter region was validated to be regulated by HSF1 under heat shock. These results provide a basis for future studies aimed at determining the mechanisms underlying thermal tolerance and provide insights into gene regulation in the Pacific oyster.
关键词	HSF1 binding site Thermal stress HSP Regulatory relationship Crassostrea gigas ChIP-seq
DOI	10.1007/s10126-019-09942-6
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000520822500002
出版者	SPRINGER
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167063
专题	实验海洋生物学重点实验室
通讯作者	Li, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Marine Fishery Inst Zhejiang Prov, Key Lab Mariculture & Enhancement Zhejiang Prov, Zhoushan 316100, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 6.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 7.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Liu, Youli,Zhu, Qihui,Li, Li,et al. Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq[J]. MARINE BIOTECHNOLOGY,2020,22(2):167-179.
APA	Liu, Youli,Zhu, Qihui,Li, Li,Wang, Wei,&Zhang, Guofan.(2020).Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq.MARINE BIOTECHNOLOGY,22(2),167-179.
MLA	Liu, Youli,et al."Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq".MARINE BIOTECHNOLOGY 22.2(2020):167-179.

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