Aberrant expression of X-linked genes RbAp46, Rsk4, and Cldn2 in breast cancer

doi:10.1158/1541-7786.MCR-06-0071

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	Aberrant expression of X-linked genes RbAp46, Rsk4, and Cldn2 in breast cancer
	Thakur, Archana ; Rahman, K. M. Wahidur ; Wu, Jack ; Bollig, Aliccia ; Biliran, Hector ; Lin, Xiukun ; Nassar, Hind ; Grignon, David J.; Sarkar, Fazlul H.; Liao, Joshua D.; Rahman, KMW, Wayne State Univ, Dept Pathol, Karmanos Canc Inst, 110 E Warren Ave, Detroit, MI 48201 USA
	2007-02-01
发表期刊	MOLECULAR CANCER RESEARCH
ISSN	1541-7786
卷号	5 期号:2 页码:171-181
文章类型	Article
摘要	The consequence of activation status or gain/loss of an X-chromosome in terms of the expression of tumor suppressor genes or oncogenes in breast cancer has not been clearly addressed. In this study, we investigated the activation status of the X-chromosomes in a panel of human breast cancer cell lines, human breast carcinoma, and adjacent mammary tissues and a panel of murine mammary epithelial sublines ranging from low to high invasive potentials. Results show that most human breast cancer cell lines were homozygous, but both benign cell lines were heterozygous for highly polymorphic X-loci (IDS and G6PD). On the other hand, 60% of human breast carcinoma cases were heterozygous for either IDS or G6PD markers. Investigation of the activation status of heterozygous cell lines revealed the presence of only one active X-chromosome, whereas most heterozygous human breast carcinoma cases had two active X-chromosomes. Furthermore, we determined whether or not an additional active X-chromosome affects expression levels of tumor suppressor genes and oncogenes. Reverse transcription-PCR data show high expression of putative tumor suppressor genes Rsk4 and RbAp46 in 47% and 79% of breast carcinoma cases, respectively, whereas Cldn2 was down-regulated in 52% of breast cancer cases compared with normal adjacent tissues. Consistent with mRNA expression, immunostaining for these proteins also showed a similar pattern. In conclusion, our data suggest that high expression of RbAp46 is likely to have a role in the development or progression of human breast cancer. The activation status of the X-chromosome may influence the expression levels of X-linked oncogenes or tumor suppressor genes.; The consequence of activation status or gain/loss of an X-chromosome in terms of the expression of tumor suppressor genes or oncogenes in breast cancer has not been clearly addressed. In this study, we investigated the activation status of the X-chromosomes in a panel of human breast cancer cell lines, human breast carcinoma, and adjacent mammary tissues and a panel of murine mammary epithelial sublines ranging from low to high invasive potentials. Results show that most human breast cancer cell lines were homozygous, but both benign cell lines were heterozygous for highly polymorphic X-loci (IDS and G6PD). On the other hand, 60% of human breast carcinoma cases were heterozygous for either IDS or G6PD markers. Investigation of the activation status of heterozygous cell lines revealed the presence of only one active X-chromosome, whereas most heterozygous human breast carcinoma cases had two active X-chromosomes. Furthermore, we determined whether or not an additional active X-chromosome affects expression levels of tumor suppressor genes and oncogenes. Reverse transcription-PCR data show high expression of putative tumor suppressor genes Rsk4 and RbAp46 in 47% and 79% of breast carcinoma cases, respectively, whereas Cldn2 was down-regulated in 52% of breast cancer cases compared with normal adjacent tissues. Consistent with mRNA expression, immunostaining for these proteins also showed a similar pattern. In conclusion, our data suggest that high expression of RbAp46 is likely to have a role in the development or progression of human breast cancer. The activation status of the X-chromosome may influence the expression levels of X-linked oncogenes or tumor suppressor genes.
关键词	Active-x Chromosome Inactivation Brca1 Mutation Ovarian-cancer High-frequency Barr Body Cells Carcinoma Kinase Components
学科领域	Oncology ; Cell Biology
DOI	10.1158/1541-7786.MCR-06-0071
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000244538200007
引用统计	被引频次：64[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1671
专题	实验海洋生物学重点实验室
通讯作者	Rahman, KMW, Wayne State Univ, Dept Pathol, Karmanos Canc Inst, 110 E Warren Ave, Detroit, MI 48201 USA
作者单位	1.Wayne State Univ, Dept Pathol, Karmanos Canc Inst, Detroit, MI 48201 USA 2.Chinese Acad Sci, Inst Oceanol, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Thakur, Archana,Rahman, K. M. Wahidur,Wu, Jack,et al. Aberrant expression of X-linked genes RbAp46, Rsk4, and Cldn2 in breast cancer[J]. MOLECULAR CANCER RESEARCH,2007,5(2):171-181.
APA	Thakur, Archana.,Rahman, K. M. Wahidur.,Wu, Jack.,Bollig, Aliccia.,Biliran, Hector.,...&Rahman, KMW, Wayne State Univ, Dept Pathol, Karmanos Canc Inst, 110 E Warren Ave, Detroit, MI 48201 USA.(2007).Aberrant expression of X-linked genes RbAp46, Rsk4, and Cldn2 in breast cancer.MOLECULAR CANCER RESEARCH,5(2),171-181.
MLA	Thakur, Archana,et al."Aberrant expression of X-linked genes RbAp46, Rsk4, and Cldn2 in breast cancer".MOLECULAR CANCER RESEARCH 5.2(2007):171-181.

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