| Structural analysis of heteropolysaccharide from Saccharina japonica and its derived oligosaccharides | |
Jin, Weihua1,2; Zhang, Wenjing1,2 ; Wang, Jing1; Ren, Sumei3; Song, Ni3; Zhang, Quanbin1; Zhang, QB
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| 2013-11-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| 卷号 | 62页码:697-704 |
| 文章类型 | Article |
| 摘要 | Degraded fucoidan (F1) was desulfated by DMSO-MeOH. And anion exchange chromatography was performed to fractionate desulfated F1 (ds-F1) into five fractions. Electrospray ionization mass spectrometry (ESI-MS) showed that each fraction contained at least one set of neutral and/or sulfated fucooligosaccharides in the form of methyl glycosides. And the structures of oligomeric fragments were characterized by ESI-CID-MS/MS and ESI-CID-MS/MS/MS. In addition, more structural features were shown by NMR. Therefore, it was concluded that LF1 contained a backbone of (1 -> 3)-linked fucopyranose residues sulfated at C-4 and branched at C-2 by fucopyranose residues and fucoglucuronomannan, fucoglucuronan, galactan and xylan were found in LF-5. Finally, it was concluded that F1 was the middle component, which contained the information of both F0.5 and F2, indicating that the differences between F1 and F0.5, F2 might be derived primarily from the different needs of algae itself. (C) 2013 Elsevier B.V. All rights reserved.; Degraded fucoidan (F1) was desulfated by DMSO-MeOH. And anion exchange chromatography was performed to fractionate desulfated F1 (ds-F1) into five fractions. Electrospray ionization mass spectrometry (ESI-MS) showed that each fraction contained at least one set of neutral and/or sulfated fucooligosaccharides in the form of methyl glycosides. And the structures of oligomeric fragments were characterized by ESI-CID-MS/MS and ESI-CID-MS/MS/MS. In addition, more structural features were shown by NMR. Therefore, it was concluded that LF1 contained a backbone of (1 -> 3)-linked fucopyranose residues sulfated at C-4 and branched at C-2 by fucopyranose residues and fucoglucuronomannan, fucoglucuronan, galactan and xylan were found in LF-5. Finally, it was concluded that F1 was the middle component, which contained the information of both F0.5 and F2, indicating that the differences between F1 and F0.5, F2 might be derived primarily from the different needs of algae itself. (C) 2013 Elsevier B.V. All rights reserved. |
| 关键词 | Esi-ms Oligosaccharides Saccharina Japonica |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1016/j.ijbiomac.2013.10.017 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000329881200103 |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; SULFATED POLYSACCHARIDES ; BROWN SEAWEEDS ; ANTICOAGULANT ACTIVITY ; CID-MS/MS ; FUCOIDAN ; DISACCHARIDES ; FUCANS ; GALACTOFUCAN ; DISSOCIATION |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16720 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Zhang, QB |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ocean Univ China, Coll Med & Pharmaceut, Qingdao 266003, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Jin, Weihua,Zhang, Wenjing,Wang, Jing,et al. Structural analysis of heteropolysaccharide from Saccharina japonica and its derived oligosaccharides[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2013,62:697-704. |
| APA | Jin, Weihua.,Zhang, Wenjing.,Wang, Jing.,Ren, Sumei.,Song, Ni.,...&Zhang, QB.(2013).Structural analysis of heteropolysaccharide from Saccharina japonica and its derived oligosaccharides.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,62,697-704. |
| MLA | Jin, Weihua,et al."Structural analysis of heteropolysaccharide from Saccharina japonica and its derived oligosaccharides".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 62(2013):697-704. |
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