Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides | |
Qi, Peng1,2,3![]() ![]() | |
2020-07-01 | |
发表期刊 | ANALYTICAL BIOCHEMISTRY
![]() |
ISSN | 0003-2697 |
卷号 | 600页码:7 |
通讯作者 | Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn) |
摘要 | In this paper, we present a facile and rapid multichannel approach for the simultaneous detection and discrimination of multiple bacterial species. The proposed assay employed four short antimicrobial peptides (SAMPs) for recognition due to their disparity in antibacterial activity against different bacterial strains, and utilized fluorescence measurements to explicate the bacterial recognition and disintegration disparity of the SAMPs. Then, linear discriminant analysis (LDA) was used to effectively discriminate and classify the observed characteristic fluorescence patterns of SAMPs towards target bacteria, exhibiting excellent bacterial discrimination and classification accuracy. This is the first report on the use of SAMPs as recognition units for simultaneous multiple bacterial detection and discrimination. The presented approach was simple, fast, highly repeatable, and required no labelling processes. According to the corresponding LDA discriminant results, six different target bacterial species could be effectively identified and discriminated within 30 min. |
关键词 | Bacterial discrimination Short antimicrobial peptides Recognition Disintegration Linear discriminant analysis |
DOI | 10.1016/j.ab.2020.113764 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[41876101] ; Young Elite Scientists Sponsorship Program by CAST[20180276] |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
WOS记录号 | WOS:000537568700012 |
出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/167640 |
专题 | 海洋环境腐蚀与生物污损重点实验室 |
通讯作者 | Qi, Peng; Zhang, Dun |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
第一作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
推荐引用方式 GB/T 7714 | Qi, Peng,Wang, Yingwen,Zhang, Dun,et al. Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides[J]. ANALYTICAL BIOCHEMISTRY,2020,600:7. |
APA | Qi, Peng,Wang, Yingwen,Zhang, Dun,Sun, Yan,&Zheng, Laibao.(2020).Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides.ANALYTICAL BIOCHEMISTRY,600,7. |
MLA | Qi, Peng,et al."Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides".ANALYTICAL BIOCHEMISTRY 600(2020):7. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Multi-emitting fluor(2953KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论