Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides
Qi, Peng1,2,3; Wang, Yingwen1,2,3,4; Zhang, Dun1,2,3; Sun, Yan1,2,3; Zheng, Laibao1,2,3,4
2020-07-01
发表期刊ANALYTICAL BIOCHEMISTRY
ISSN0003-2697
卷号600页码:7
通讯作者Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
摘要In this paper, we present a facile and rapid multichannel approach for the simultaneous detection and discrimination of multiple bacterial species. The proposed assay employed four short antimicrobial peptides (SAMPs) for recognition due to their disparity in antibacterial activity against different bacterial strains, and utilized fluorescence measurements to explicate the bacterial recognition and disintegration disparity of the SAMPs. Then, linear discriminant analysis (LDA) was used to effectively discriminate and classify the observed characteristic fluorescence patterns of SAMPs towards target bacteria, exhibiting excellent bacterial discrimination and classification accuracy. This is the first report on the use of SAMPs as recognition units for simultaneous multiple bacterial detection and discrimination. The presented approach was simple, fast, highly repeatable, and required no labelling processes. According to the corresponding LDA discriminant results, six different target bacterial species could be effectively identified and discriminated within 30 min.
关键词Bacterial discrimination Short antimicrobial peptides Recognition Disintegration Linear discriminant analysis
DOI10.1016/j.ab.2020.113764
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[41876101] ; Young Elite Scientists Sponsorship Program by CAST[20180276]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical
WOS记录号WOS:000537568700012
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/167640
专题海洋环境腐蚀与生物污损重点实验室
通讯作者Qi, Peng; Zhang, Dun
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
推荐引用方式
GB/T 7714
Qi, Peng,Wang, Yingwen,Zhang, Dun,et al. Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides[J]. ANALYTICAL BIOCHEMISTRY,2020,600:7.
APA Qi, Peng,Wang, Yingwen,Zhang, Dun,Sun, Yan,&Zheng, Laibao.(2020).Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides.ANALYTICAL BIOCHEMISTRY,600,7.
MLA Qi, Peng,et al."Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides".ANALYTICAL BIOCHEMISTRY 600(2020):7.
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