Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The immune function of a novel crustin with an atypical WAP domain in regulating intestinal microbiota homeostasis in Litopenaeus vannamei | |
Lv, Xinjia1,2,3,4 ; Li, Shihao1,2,4; Yu, Yang1,2,4; Xiang, Jianhai1,2; Li, Fuhua1,2,4,5
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| 2020-10-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| ISSN | 0145-305X |
| 卷号 | 111页码:11 |
| 摘要 | Crustins are a family of antimicrobial peptides (AMP) with multiple functions, including antimicrobial activity, capability of protease inhibition, phagocytosis promotion, and wound healing in crustaceans. Till present, several members of crustins have been identified and their activities were studied. However, there are still less investigations on how they play functions in vivo. Here, we identified a novel crustin with an atypical WAP domain, LvCrustin I-1, which is mainly distributed in tissues, including intestine, gill, epidermis and stomach of the shrimp Litopenaeus vannamei. The expression level of LvCrustin I-1 was significantly up-regulated at 3 h, 6 h, 12 h, and 24 h after Vibrio parahaemolyticus infection. Knockdown of LvCrustin I-1 with dsRNA resulted in a significant increase of the bacteria number in hepatopancreas of shrimp upon V. parahaemolyticus infection, showing that LvCrustin I-1 participated in pathogen infection process. Recombinant LvCrustin I-1 protein showed microorganism-binding activity rather than antibacterial activity against tested bacteria. Furthermore, significant difference existed between the intestinal microbiota in shrimp before and after LvCrustin I-1 knockdown based on the result of alpha and NMDS analyses. Knockdown of LvCrustin I-1 increased the proportion of Demequina, Nautella, Propionibacterium, Anaerospora and decreased the proportion of Bacteroidia and Vibrio. These data suggest that LvCrustin I-1 might perform its immunological function through modulation of the intestinal microbiota homeostasis rather than direct inhibition of bacterial growth in shrimp. |
| 关键词 | Antimicrobial peptide Crustin Intestinal microbiota Homeostasis Crustacean |
| DOI | 10.1016/j.dci.2020.103756 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[41776158] ; China Agricultural Research System[CARS-48] |
| WOS研究方向 | Fisheries ; Immunology ; Zoology |
| WOS类目 | Fisheries ; Immunology ; Zoology |
| WOS记录号 | WOS:000551873700004 |
| 出版者 | ELSEVIER SCI LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167994 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Li, Shihao; Li, Fuhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Lv, Xinjia,Li, Shihao,Yu, Yang,et al. The immune function of a novel crustin with an atypical WAP domain in regulating intestinal microbiota homeostasis in Litopenaeus vannamei[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,111:11. |
| APA | Lv, Xinjia,Li, Shihao,Yu, Yang,Xiang, Jianhai,&Li, Fuhua.(2020).The immune function of a novel crustin with an atypical WAP domain in regulating intestinal microbiota homeostasis in Litopenaeus vannamei.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,111,11. |
| MLA | Lv, Xinjia,et al."The immune function of a novel crustin with an atypical WAP domain in regulating intestinal microbiota homeostasis in Litopenaeus vannamei".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 111(2020):11. |
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| The immune function （3572KB） | 期刊论文 | 作者接受稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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