Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives

doi:10.3389/fbioe.2020.00833

IOCAS-IR

	Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives
	Sun, Shenmei 1,2; Ding, Yamei 3; Liu, Min 1; Xian, Mo 1; Zhao, Guang 1,4
	2020-07-23
发表期刊	FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
ISSN	2296-4185
卷号	8 页码:13
通讯作者	Zhao, Guang(zhaoguang@sdu.edu.cn)
摘要	Poly(3-hydroxybutyrate) (PHB) is a biodegradable and biocompatible thermoplastic, and synthesized from the central metabolite acetyl-CoA. The acetyl-CoA synthesis from glucose presents low atomic economy due to the release of CO(2)in pyruvate decarboxylation. As ethanol and acetate can be converted into acetyl-CoA directly, they were used as carbon source for PHB production in this study. The reductase mutant AdhE A267T/E568K was introduced intoEscherichia colito enable growth on ethanol, and acetate utilization was improved by overexpression of acetyl-CoA synthetase ACS. Comparison of the PHB production using glucose, ethanol or acetate as sole carbon source showed that the production and yield from ethanol was much higher than those from glucose and acetate, and metabolome analysis revealed the differences in metabolism of glucose, ethanol and acetate. Furthermore, other acetyl-CoA derived chemicals including 3-hydroxypropionate and phloroglucinol were produced from those three feedstocks, and similar results were achieved, suggesting that ethanol could be a suitable carbon source for the production of acetyl-CoA derivatives.
关键词	carbon source glucose ethanol acetate acetyl-CoA bioproduction
DOI	10.3389/fbioe.2020.00833
收录类别	SCI
语种	英语
资助项目	NSFC[31722001] ; NSFC[31670089] ; Natural Science Foundation of Shandong Province[JQ201707] ; National Defense Science and Technology Innovation Zone Foundation of China ; Defense Industrial Technology Development Program[JCKY2018130B005] ; Shandong University
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
WOS类目	Biotechnology & Applied Microbiology ; Multidisciplinary Sciences
WOS记录号	WOS:000560293700001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：32[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168187
专题	中国科学院海洋研究所
通讯作者	Zhao, Guang
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Shandong Univ, State Key Lab Microbial Technol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Sun, Shenmei,Ding, Yamei,Liu, Min,et al. Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2020,8:13.
APA	Sun, Shenmei,Ding, Yamei,Liu, Min,Xian, Mo,&Zhao, Guang.(2020).Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8,13.
MLA	Sun, Shenmei,et al."Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2020):13.

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