A preliminary genetic map of Zhikong scallop (Chlamys farreri Jones et Preston 1904)

doi:10.1111/j.1365-2109.2005.01268.x

IOCAS-IR > 实验海洋生物学重点实验室

	A preliminary genetic map of Zhikong scallop (Chlamys farreri Jones et Preston 1904)
	Wang, LL ; Song, LS ; Chang, YQ ; Xu, W ; Ni, DJ ; Guo, XM ; Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
	2005-05-13
发表期刊	AQUACULTURE RESEARCH
ISSN	1355-557X
卷号	36 期号:7 页码:643-653
文章类型	Article
摘要	Zhikong scallop (Chlamys farreri Jones et Preston 1904) is one of the most important aquaculture species in China. The development of a genetic linkage map would provide a powerful tool for the genetic improvement of this species. Amplified fragment length polymorphism (AFLP) is a PCR-based technique that has proven to be powerful in genome fingerprinting and mapping, and population analysis. Genetic maps of C. farreri were constructed using AFLP markers and a full-sib family with 60 progeny. A total of 503 segregating AFLP markers were obtained, with 472 following the Mendelian segregation ratio of 1:1 and 31 markers showing significant (P< 0.05) segregation distortion. The male map contained 166 informative AFLP markers in 23 linkage groups covering 2468 cM. The average distance between markers was 14.9 cM. The female genetic map consisted of 198 markers in 25 linkage groups spanning 3130 cM with an average inter-marker spacing of 15.8 cM. DNA polymorphisms that segregated in a 3:1 ratio as well as the AFLP markers that were heterozygous in both parents were included to construct combined linkage genetic map. Five shared linkage groups, ranging from 61.1 to 162.5 cM, were identified between the male and female maps, covering 431 cM. Amplified fragment length polymorphism markers appeared to be evenly distributed within the linkage groups. Although preliminary, these maps provide a starting point for the mapping of the functional genes and quantitative trait loci in C. farreri.; Zhikong scallop (Chlamys farreri Jones et Preston 1904) is one of the most important aquaculture species in China. The development of a genetic linkage map would provide a powerful tool for the genetic improvement of this species. Amplified fragment length polymorphism (AFLP) is a PCR-based technique that has proven to be powerful in genome fingerprinting and mapping, and population analysis. Genetic maps of C. farreri were constructed using AFLP markers and a full-sib family with 60 progeny. A total of 503 segregating AFLP markers were obtained, with 472 following the Mendelian segregation ratio of 1:1 and 31 markers showing significant (P< 0.05) segregation distortion. The male map contained 166 informative AFLP markers in 23 linkage groups covering 2468 cM. The average distance between markers was 14.9 cM. The female genetic map consisted of 198 markers in 25 linkage groups spanning 3130 cM with an average inter-marker spacing of 15.8 cM. DNA polymorphisms that segregated in a 3:1 ratio as well as the AFLP markers that were heterozygous in both parents were included to construct combined linkage genetic map. Five shared linkage groups, ranging from 61.1 to 162.5 cM, were identified between the male and female maps, covering 431 cM. Amplified fragment length polymorphism markers appeared to be evenly distributed within the linkage groups. Although preliminary, these maps provide a starting point for the mapping of the functional genes and quantitative trait loci in C. farreri.
关键词	Chlamys Farreri Genetic Map Aflp Scallop Aquaculture
学科领域	Fisheries
DOI	10.1111/j.1365-2109.2005.01268.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000228866900004
引用统计	被引频次：40[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1685
专题	实验海洋生物学重点实验室
通讯作者	Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Dalian Fisheries Coll, Dalian, Peoples R China 4.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ USA
推荐引用方式 GB/T 7714	Wang, LL,Song, LS,Chang, YQ,et al. A preliminary genetic map of Zhikong scallop (Chlamys farreri Jones et Preston 1904)[J]. AQUACULTURE RESEARCH,2005,36(7):643-653.
APA	Wang, LL.,Song, LS.,Chang, YQ.,Xu, W.,Ni, DJ.,...&Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2005).A preliminary genetic map of Zhikong scallop (Chlamys farreri Jones et Preston 1904).AQUACULTURE RESEARCH,36(7),643-653.
MLA	Wang, LL,et al."A preliminary genetic map of Zhikong scallop (Chlamys farreri Jones et Preston 1904)".AQUACULTURE RESEARCH 36.7(2005):643-653.

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