iTRAQ-based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress

doi:10.1111/are.14880

IOCAS-IR > 实验海洋生物学重点实验室

	iTRAQ-based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress
	Liu, Lijie 1,2,3; Lin, Lidong 3; Ma, Zengling 4; Wang, Guangce 1; Wu, Mingjiang 4
	2020-09-20
发表期刊	AQUACULTURE RESEARCH
ISSN	1355-557X
页码	11
通讯作者	Wang, Guangce(gcwang@qdio.ac.cn)
摘要	Global warming increases seawater temperature, causing high temperature stress to marine organisms, including algae. This study aimed to explore the global proteomic response ofSargassum fusiformeunder high temperature stress.Sargassum fusiformeseedlings were cultured in natural seawater for 24 hr and subjected to different temperatures (22 degrees C, control group; 27 degrees C and 32 degrees C, high temperature stress group) for 1, 3, 5 and 7 days. Changes in their membrane lipid peroxidation after high temperature stress were investigated. Proteomic changes in the air bladders ofS. fusiformewere analysed using isobaric tags for relative and absolute quantification, along with liquid chromatography-tandem mass spectrometry. Data were analysed using bioinformatics methods. Results showed that high temperature stress destroyed the cell membrane of the air bladders. Further, 28 and 53 differentially expressed proteins (DEPs) were found in the 27 degrees C and 32 degrees C treatment groups respectively. These DEPs were mainly involved in glycolysis, single-organism catabolism, purine nucleoside diphosphate metabolism and carbohydrate catabolism. In addition, DEPs were significantly enriched in 10 pathways, such as glycolytic process, biosynthesis of antibiotics, ribosome, biosynthesis of secondary metabolites and biosynthesis of amino acids. Proteomics analyses indicated that proteins associated with synthesis, folding, degradation, photosynthesis and energy and carbohydrate metabolism are differentially expressed under high temperature stress and normal conditions.
关键词	high temperature iTRAQ proteomics Sargassum fusiforme
DOI	10.1111/are.14880
收录类别	SCI
语种	英语
资助项目	Major Fisheries and Agriculture Science and Technology Projects in Dongtou District of Wenzhou City[N2018Y03A] ; Science and Technology Plan Projects in Dongtou District of Wenzhou City[N2016Y20B]
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:000571665900001
出版者	WILEY
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168808
专题	实验海洋生物学重点实验室
通讯作者	Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qiqihar Univ, Coll Life Sci & Agr Forestry, Qiqihar, Heilongjiang, Peoples R China 3.Marine & Fishery Dev Res Ctr Dongtou Dist Wenzhou, Wenzhou, Zhejiang, Peoples R China 4.Wenzhou Univ, Coll Life & Environm Sci, Chashan Univ Town, Wenzhou, Zhejiang, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Lijie,Lin, Lidong,Ma, Zengling,et al. iTRAQ-based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress[J]. AQUACULTURE RESEARCH,2020:11.
APA	Liu, Lijie,Lin, Lidong,Ma, Zengling,Wang, Guangce,&Wu, Mingjiang.(2020).iTRAQ-based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress.AQUACULTURE RESEARCH,11.
MLA	Liu, Lijie,et al."iTRAQ-based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress".AQUACULTURE RESEARCH (2020):11.

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