Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei

doi:10.1016/j.fsi.2020.07.014

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei
	Lv, Xinjia1,2,3 ; Li, Shihao 1,2,4; Yu, Yang1,2,4 ; Zhang, Xiaojun 1,2,4; Li, Fuhua 1,2,4,5
	2020-10-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	105 页码:393-404
通讯作者	Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
摘要	Gills as the respiratory tissue of crustacean encounter various threats in the environment. The immune defense in gills is essential to the health of shrimp. In the present study, we identified a novel gill-abundant crustin, LvCrustin II-1, from the shrimp Litopenaeus vannamei. The full-length open reading frame of LvCrustin II-1 is 522 bp, which encodes 173 amino acid residues. The amino acid sequence of LvCrustin II-1 contained a signal peptide, a glycine rich region, a cysteine rich region and a whey acidic protein (WAP) domain. The expression level of LvCrustin II-1 was significantly up-regulated at different time points after Vibrio parahaemolyticus immersion. When LvCrustin II-1 was silenced by dsRNA interference, the count of bacteria colonies increased significantly in the hepatopancreas of shrimp after V. parahaemolyticus immersion, which indicated that the infection progress of pathogenic bacteria was accelerated after LvCrustin II-1 knockdown. Compared with the microbiota of seawater, the lower proportion of aquatic bacteria and higher proportion of symbiont in the gills microbiota of shrimp indicated the bacterial colonization was modulated by the host. Knockdown of LvCrustin II-1 changed the proportion of some potential pathogens and aquatic bacteria, which supported the idea that the new identified crustin in the gills played important roles in modulation of the microbiota community in shrimp. The present data provided new insights into the multiple functions of crustin in the immunity of shrimp.
关键词	Antimicrobial peptides Crustin RNA interference Gill microbiota Litopenaeus vannamei
DOI	10.1016/j.fsi.2020.07.014
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[41776158] ; China Agricultural Research System[CARS-48]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000571746900005
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168811
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Lv, Xinjia,Li, Shihao,Yu, Yang,et al. Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2020,105:393-404.
APA	Lv, Xinjia,Li, Shihao,Yu, Yang,Zhang, Xiaojun,&Li, Fuhua.(2020).Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,105,393-404.
MLA	Lv, Xinjia,et al."Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 105(2020):393-404.

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