Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics

doi:10.1016/j.bios.2020.112953

IOCAS-IR

	Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics
	Song, Fengge1 ; Shen, Yuanyuan 1; Wei, Yangdao 1; Yang, Chunrong 2; Ge, Xiaolin 1; Wang, Aimin 1; Li, Chaoyang 1; Wan, Yi 1,3; Li, Jinghong 2
	2021-03-15
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	176 页码:8
通讯作者	Wan, Yi(993602@hainanu.edu.cn)
摘要	The rapid identification of pathogenic microorganisms plays a crucial role in the timely diagnosis and treatment strategies during a global pandemic, especially in resource-limited area. Herein, we present a sensitive biosensor strategy depended on botulinum neurotoxin type A light chain (BoNT/A LC) activated complex assay (BACA). BoNT/A LC, the surrogate of BoNT/A which embodying the most potent biological poisons, could serve as an ultrasensitive signal reporter with high signal-to-noise ratio to avoid common strong background response, poor stability and low intensity of current biosensor methods. A nanoparticle hybridization system, involving specific binding probes that recognize pathogenic 16S rRNAs or SARS-CoV-2 gene site, was developed to measure doublestranded biotinylated target DNA containing a single-stranded overhang using Fluorescence Resonance Energy Transfer (FRET)-based assay and colorimetric method. The method is validated widely by six different bacteria strains and severe acute respiratory related coronavirus 2 (SARS-CoV-2) nucleic acid, demonstrating a single cell or 1 aM nucleic acid detecting sensitivity. This detection strategy offers a solution for general applications and has a great prospect to be a simple instrument-free colorimetric tool, especially when facing public health emergency.
关键词	Bacterial diagnostic SARS-CoV-2 Botulinum toxin Fluorescence resonance energy transfer Colorimetric detection Biosensors
DOI	10.1016/j.bios.2020.112953
收录类别	SCI
语种	英语
资助项目	Key Research and the Development Program of Hainan, China[ZDYF(XGFY)2020002] ; National Key Research and Development Program of China, China[2018YFD0900704] ; National Natural Science Foundation of China, China[41866002] ; Tsinghua University Initiative Scientific Research Program, China[2020Z99CFZ019] ; Shandong Key Laboratory of Corrosion Science, China[KLCS201910] ; Research Foundation of Hainan University, China[KYQD(ZR)1711]
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号	WOS:000612675000002
出版者	ELSEVIER ADVANCED TECHNOLOGY
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169734
专题	中国科学院海洋研究所
通讯作者	Wan, Yi
作者单位	1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Key Lab Trop Biol Resources, Marine Coll,Minist Educ,Sch Life & Pharmaceut Sci, 56 Renmin Rd, Haikou 570228, Hainan, Peoples R China 2.Tsinghua Univ, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Dept Chem, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Shandong Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Song, Fengge,Shen, Yuanyuan,Wei, Yangdao,et al. Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics[J]. BIOSENSORS & BIOELECTRONICS,2021,176:8.
APA	Song, Fengge.,Shen, Yuanyuan.,Wei, Yangdao.,Yang, Chunrong.,Ge, Xiaolin.,...&Li, Jinghong.(2021).Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics.BIOSENSORS & BIOELECTRONICS,176,8.
MLA	Song, Fengge,et al."Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics".BIOSENSORS & BIOELECTRONICS 176(2021):8.

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